Anticorps Monoclonal anti-CD86

CD86 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(1)

CloneNo.

GL1

N° de cat : CL488-65068

Synonymes

B7, B7 2, B7.2, B70, B7-2

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Galerie de données de validation

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  FC experiment of C57BL/6 mouse splenocytes using CL488-65068

  1X10^6 unstimulated or LPS stimulated (5 ug/mL, 3 days) C57BL/6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD86 (CL488-65068, Clone: GL1). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: LPS treated C57BL/6 mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

CL488-65068 cible CD86 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes B de rate de souris CBA/Ca activées par LPS
• Nom complet: CD86 antigen
• Numéro d’acquisition GenBank: BC013807
• Symbole du gène: Cd86
• Identification du gène (NCBI): 12524
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publications

Species	Application	Title
mouse	FC	Respir Res; YTHDF1-mediated m6A modification of GBP4 promotes M1 macrophage polarization in acute lung injury; Authors - Fengan Cao

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