Anticorps Monoclonal anti-CD86

CD86 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

IF, FC

Conjugaison

PE Fluorescent Dye

Publications(18)

CloneNo.

GL1

N° de cat : PE-65068

Synonymes

B7, B7 2, B7.2, B70, B7-2

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Galerie de données de validation

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  FC experiment of mouse splenocytes using PE-65068

  1X10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD86 (PE-65068, Clone: GL1). Cells were not fixed.

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  FC experiment of mouse splenocytes using PE-65068

  1X10^6 untreated (dashed line) or LPS-treated mouse splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD86 (PE-65068, Clone: GL1) (red), or 0.2 ug Isotype Control (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: LPS treated mouse splenocytes,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 3 publications below
• FC: See 13 publications below

Informations sur le produit

PE-65068 cible CD86 dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes B de rate de souris CBA/Ca activées par LPS
• Nom complet: CD86 antigen
• Numéro d’acquisition GenBank: BC013807
• Symbole du gène: Cd86
• Identification du gène (NCBI): 12524
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publications

Species	Application	Title
mouse	FC	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
mouse	FC	ACS Appl Mater Interfaces; Dual-Regulated Biomimetic Nanocomposites For Promoted Tumor Photodynamic Immunotherapy; Authors - Li Liao
mouse	FC	Int J Nanomedicine; Unexpected Inhibitory Role of Silica Nanoparticles on Lung Cancer Development by Promoting M1 Polarization of Macrophages; Authors - Meng Xiang
	FC	J Integr Neurosci; Avicularin Treatment Ameliorates Ischemic Stroke Damage by Regulating Microglia Polarization and its Exosomes via the NLRP3 Pathway; Authors - Yan Shi
mouse	FC	Naunyn Schmiedebergs Arch Pharmacol; Akkermansia muciniphila relieves inflammatory response in DSS-induced ulcerative colitis in mice through regulating macrophage polarization via SCFAs-SLC52A2/FFAR2 pathway; Authors - Lin Zhang
mouse	IF	Cytokine; Extracellular HSP70 facilitated β-glucan induced trained immunity in macrophages to suppress sepsis via TLR2-NF-κB axis; Authors - Ran Qi

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