Anticorps Polyclonal de lapin anti-CDC25B

CDC25B Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF

Conjugaison

Non conjugué

Publications

2

N° de cat : 10644-1-AP

Synonymes

CDC25B, CDC25HU2, M phase inducer phosphatase 2

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Galerie de données de validation

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  WB analysis of mouse liver using 10644-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10644-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10644-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10644-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10644-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10644-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10644-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10644-1-AP (CDC25B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10644-1-AP

  IP Result of anti-CDC25B (IP:10644-1-AP, 4ug; Detection:10644-1-AP 1:300) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human lymphoma using 10644-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10644-1-AP (CDC25B antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules Jurkat, cellules Raji, tissu cérébral de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

10644-1-AP cible CDC25B dans les applications de WB, IP, IHC, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDC25B Protéine recombinante Ag0994
• Nom complet: cell division cycle 25 homolog B (S. pombe)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC006395
• Symbole du gène: CDC25B
• Identification du gène (NCBI): 994
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B).

Publications

Species	Application	Title
mouse	WB, IF	BMC Dev Biol; Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation.; Authors - Santanu De
mouse	IF	BMC Dev Biol; YWHA (14-3-3) protein isoforms and their interactions with CDC25B phosphatase in mouse oogenesis and oocyte maturation.; Authors - Alaa A Eisa