Anticorps Monoclonal anti-Chromogranin A

Chromogranin A Monoclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

10

CloneNo.

3H11C11

N° de cat : 60135-1-Ig

Synonymes

CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2

---

Galerie de données de validation

• 

  IHC staining of human pancreas using 60135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60135-1-Ig (Chromogranin A antibody) at dilution of 1:80000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 60135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60135-1-Ig (Chromogranin A antibody) at dilution of 1:80000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60135-1-Ig (Chromogranin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60135-1-Ig (Chromogranin A antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 60135-1-Ig(Chromogranin A antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 60135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 60135-1-Ig(Chromogranin A antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of PC-12 using 60135-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using 60135-1-Ig (Chromogranin A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en IHC: tissu pancréatique humain, tissu de cancer du poumon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-12,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:5000-1:80000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

60135-1-Ig cible Chromogranin A dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Chromogranin A Protéine recombinante Ag0807
• Nom complet: chromogranin A (parathyroid secretory protein 1)
• Masse moléculaire calculée: 51 kDa
• Numéro d’acquisition GenBank: BC006459
• Symbole du gène: CHGA
• Identification du gène (NCBI): 1113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Publications

Species	Application	Title
mouse	IF	Mol Metab; Genetic activation of α-cell glucokinase in mice causes enhanced glucose-suppression of glucagon secretion during normal and diabetic states.; Authors - Varun Bahl
human	WB,IHC	J Pathol; Histone demethylase PHF8 drives neuroendocrine prostate cancer progression by epigenetically upregulating FOXA2.; Authors - Qiuli Liu
	IF	iScience; Efficient Generation and Transcriptomic Profiling of Human iPSC-Derived Pulmonary Neuroendocrine Cells.; Authors - Pooja Hor
	WB	Sci Rep; PMID: 31197210; Authors - Trine Maxel
human	WB	Sci Rep; Cancer cell's neuroendocrine feature can be acquired through cell-cell fusion during cancer-neural stem cell interaction.; Authors - Liyuan Yin
human	IF	Transl Oncol; High throughput proteomic and metabolic profiling identified target correction of metabolic abnormalities as a novel therapeutic approach in head and neck paraganglioma.; Authors - Zhigang Wang