Anticorps Polyclonal de lapin anti-CHRNA7

CHRNA7 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 21379-1-AP

Synonymes

CHRNA7, CHRNA7 2, NACHRA7

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Galerie de données de validation

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  WB analysis using 21379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 21379-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 21379-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21379-1-AP (CHRNA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21379-1-AP (CHRNA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21379-1-AP (CHRNA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21379-1-AP (CHRNA7 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 21379-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21379-1-AP (CHRNA7 Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of SH-SY5Y using 21379-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug CHRNA7 antibody (21379-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HL-60, cellules MCF-7, cellules SH-SY5Y, tissu cérébral de rat, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Informations sur le produit

21379-1-AP cible CHRNA7 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHRNA7 Protéine recombinante Ag15980
• Nom complet: cholinergic receptor, nicotinic, alpha 7
• Masse moléculaire calculée: 502 aa, 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC037571
• Symbole du gène: CHRNA7
• Identification du gène (NCBI): 1139
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin.

Publications

Species	Application	Title
mouse	WB	J Neuroinflammation; Kynurenic acid blunts A1 astrocyte activation against neurodegeneration in HIV-associated neurocognitive disorders; Authors - Jingxian Lun
human	WB	J Invest Dermatol; Chrna5 is overexpressed in psoriasis patients and promotes psoriasis-like inflammation in mouse models.; Authors - Jing Wang
mouse	WB,IF	Front Pharmacol; Autophagy is Involved in Neuroprotective Effect of Alpha7 Nicotinic Acetylcholine Receptor on Ischemic Stroke.; Authors - Zhe-Qi Xu
rat	WB,IF	Front Pharmacol; Activation of α7nAChR Protects Against Gastric Inflammation and Dysmotility in Parkinson's Disease Rats.; Authors - Li Zhou
mouse	WB	J Alzheimers Dis; Gx-50 Inhibits Neuroinflammation via α7 nAChR Activation of the JAK2/STAT3 and PI3K/AKT Pathways; Authors - Shi Shi
human,mouse	WB,IF	Mol Med; α7 nicotinic acetylcholine receptor agonist GTS-21 attenuates DSS-induced intestinal colitis by improving intestinal mucosal barrier function.; Authors - Ziping Ye

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