Anticorps Polyclonal de lapin anti-CRH/CRF

CRH/CRF Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

25

Avis (2)

N° de cat : 10944-1-AP

Synonymes

Corticoliberin, CRF, CRH

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Galerie de données de validation

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  WB analysis of human brain using 10944-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10944-1-AP (CRH/CRF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10944-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10944-1-AP (CRH/CRF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10944-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10944-1-AP (CRH/CRF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10944-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10944-1-AP (CRH/CRF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10944-1-AP (CRH/CRF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10944-1-AP (CRH/CRF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 10944-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CRH/CRF antibody (10944-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 10944-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CRH/CRF antibody (10944-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 11 publications below
• IF: See 11 publications below

Informations sur le produit

10944-1-AP cible CRH/CRF dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris, Microtus ochrogaster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRH/CRF Protéine recombinante Ag1371
• Nom complet: CRH/CRF
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC011031
• Symbole du gène: CRH
• Identification du gène (NCBI): 1392
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.

Publications

Species	Application	Title
Microtus Ochrogaster	WB	Neurobiol Stress; Social isolation alters behavior, the gut-immune-brain axis, and neurochemical circuits in male and female prairie voles.; Authors - Meghan Donovan
human	IHC,IF	Elife; Single-cell transcriptome analysis identifies a unique tumor cell type producing multiple hormones in ectopic ACTH and CRH secreting pheochromocytoma.; Authors - Xuebin Zhang
human	IHC,IF	Brain Behav Immun; Defects in NLRP6, autophagy and goblet cell homeostasis are associated with reduced duodenal CRH receptor 2 expression in patients with functional dyspepsia.; Authors - Jessica K Bruce
human	IF	Int J Mol Sci; Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa.; Authors - Mika Yamanaka-Takaichi
mouse	IHC	Development; PLXNA1 and PLXNA3 cooperate to pattern the nasal axons that guide gonadotropin-releasing hormone neurons.; Authors - Roberto Oleari
zebrafish	WB, IF	Neuropharmacology; PMID: 25842247; Authors - Fengjiao Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

roberto (Verified Customer) (06-07-2019)

High specific signal

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: postnatal mouse brain

CRH/CRF Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in postnatal mouse brain (Cat no:10944-1-AP)

FH

Yan (Verified Customer) (10-31-2017)

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: human postmortem brain

CRH/CRF Antibody Western Blot validation (1:10000 dilution) in human postmortem brain (Cat no:10944-1-AP)

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