Anticorps Polyclonal de lapin anti-DLL4

• Phare
• Validé par KD/KO

DLL4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 21584-1-AP

Synonymes

delta like 4 (Drosophila), Delta like protein 4, Delta4, DLL4, Drosophila Delta homolog 4, hdelta2

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Galerie de données de validation

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  WB analysis using 21584-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21584-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 21584-1-AP

  IP result of anti-DLL4 (IP:21584-1-AP, 4ug; Detection:21584-1-AP 1:300) with mouse lung tissue lysate 4000 ug.

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  IHC staining of mouse lung using 21584-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21584-1-AP (DLL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 21584-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21584-1-AP (DLL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human stomach cancer using 21584-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using 21584-1-AP (DLL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules A431, cellules bEnd.3, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris,
• Résultats positifs en IHC: tissu pulmonaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer de l'estomac humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

21584-1-AP cible DLL4 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DLL4 Protéine recombinante Ag16140
• Nom complet: delta-like 4 (Drosophila)
• Masse moléculaire calculée: 685 aa, 75 kDa
• Poids moléculaire observé: 75-80 kDa
• Numéro d’acquisition GenBank: BC106950
• Symbole du gène: DLL4
• Identification du gène (NCBI): 54567
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DLL4, also named as Delta4, plays a role in the Notch signaling pathway. It activates Notch-1 and Notch-4. Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. DLL4 is highly expressed in the vascular endothelium and is involved in angiogenesis.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
mouse	WB,IF	Angiogenesis; Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes.; Authors - Jing Bai
human	WB	Bioact Mater; Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid.; Authors - Wenjing Liu
mouse	WB	Theranostics; Endothelial PERK-ATF4-JAG1 axis activated by T-ALL remodels bone marrow vascular niche.; Authors - Cui Liu
human	WB	EMBO Rep; Dysregulation of the miR-30c/DLL4 axis by circHIPK3 is essential for KSHV lytic replication.; Authors - Katherine L Harper ; KO Validated

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