Anticorps Polyclonal de lapin anti-GEFT-Specific

GEFT-Specific Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(14)

Avis (1)

N° de cat : 14839-1-AP

Synonymes

GEFT, ARHGEF25, RhoA/RAC/CDC42 exchange factor, Rho guanine nucleotide exchange factor 25, p63RhoGEF

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Galerie de données de validation

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Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

14839-1-AP cible GEFT-Specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: RhoA/RAC/CDC42 exchange factor
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: NM_182947
• Symbole du gène: GEFT
• Identification du gène (NCBI): 115557
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68 kDa).

Publications

Species	Application	Title
mouse	WB	Genes Dev; TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway.; Authors - Kant Shashi S
human	WB	Circ Res; p63RhoGEF couples Gα(q/11)-mediated signaling to Ca2+ sensitization of vascular smooth muscle contractility.; Authors - Momotani Ko K
mouse	WB	Sci Signal; RSK2 contributes to myogenic vasoconstriction of resistance arteries by activating smooth muscle myosin and the Na+/H+ exchanger.; Authors - Mykhaylo V Artamonov
rat	WB,IHC	FASEB J; p63RhoGEF--a key mediator of angiotensin II-dependent signaling and processes in vascular smooth muscle cells.; Authors - Wuertz Christina M CM
mouse	WB,IHC,IF	Mol Cell Biol; Modulation of muscle regeneration, myogenesis, and adipogenesis by the Rho family guanine nucleotide exchange factor GEFT.; Authors - Bryan Brad A BA
human	WB	J Hypertens; Increased level of p63RhoGEF and RhoA/Rho kinase activity in hypertensive patients.; Authors - Lorenzo A Calò

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joelle (Verified Customer) (09-10-2019)

Overnight at 4 degrees

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HHL16

GEFT-Specific Antibody Western Blot, validation (1/1000 dilution) in HHL16 (Cat no:14839-1-AP)

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