Anticorps Polyclonal de lapin anti-GSK3B

• Phare
• Validé par KD/KO

GSK3B Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 15113-1-AP

Synonymes

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta

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Galerie de données de validation

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  WB analysis of A549 using 15113-1-AP

  WB result of GSK3B antibody (15113-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSK3B transfected A549 cells.

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  WB analysis of mouse ovary using 15113-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15113-1-AP (GSK3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15113-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15113-1-AP (GSK3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15113-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15113-1-AP (GSK3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15113-1-AP

  IP Result of anti-GSK3B (IP:15113-1-AP, 4ug; Detection:15113-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human testis using 15113-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15113-1-AP (GSK3B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15113-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15113-1-AP (GSK3B Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu ovarien de souris, cellules A549, cellules HEK-293, cellules HeLa
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below

Informations sur le produit

15113-1-AP cible GSK3B dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSK3B Protéine recombinante Ag7101
• Nom complet: glycogen synthase kinase 3 beta
• Masse moléculaire calculée: 433 aa, 48 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC000251
• Symbole du gène: GSK3B
• Identification du gène (NCBI): 2932
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the 2 isoforms of GSK3B.

Publications

Species	Application	Title
human	WB	Front Oncol; The Role of miR-640: A Potential Suppressor in Breast Cancer via Wnt7b/β-catenin Signaling Pathway.; Authors - Chun Tang
human	WB	J Ethnopharmacol; Entada phaseoloides extract suppresses hepatic gluconeogenesis via activation of the AMPK signaling pathway.; Authors - Tao Zheng
mouse	WB	Biochem Biophys Res Commun; Perilipin 5 ameliorates high-glucose-induced podocyte injury via Akt/GSK-3β/Nrf2-mediated suppression of apoptosis, oxidative stress, and inflammation.; Authors - Jie Feng
human	WB	Biochem Biophys Res Commun; NOL4L, a novel nuclear protein, promotes cell proliferation and metastasis by enhancing the PI3K/AKT pathway in ovarian cancer.; Authors - Feikai Lin
human	WB	Microvasc Res; Luteolin alleviates ischemia/reperfusion injury-induced no-reflow by regulating Wnt/β-catenin signaling in rats.; Authors - Xichun Qin
human	WB	Cell Biol Int; Identification and analysis of glycogen synthase kinase 3 beta1 interactome.; Authors - Gao Xuejuan X