Anticorps Polyclonal de lapin anti-HLA-F

HLA-F Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB,ELISA

Conjugaison

Non conjugué

N° de cat : 55379-1-AP

Synonymes

CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F

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Galerie de données de validation

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  WB analysis of Raji using 55379-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 55379-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 55379-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55379-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55379-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55379-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules A431, cellules Jurkat, tissu placentaire humain, tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

55379-1-AP cible HLA-F dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: major histocompatibility complex, class I, F
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa, 66-70 kDa
• Numéro d’acquisition GenBank: NM_018950
• Symbole du gène: HLA-F
• Identification du gène (NCBI): 3134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)