- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-IFNAR1
IFNAR1 Polyclonal Antibody for FC, WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris
Applications
WB, IF, FC, ELISA
Conjugaison
Non conjugué
N° de cat : 13083-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules Jurkat, cellule k-562, tissu hépatique de souris |
| Résultats positifs en cytométrie | cellules K-562 |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IF | See 1 publications below |
Informations sur le produit
13083-1-AP cible IFNAR1 dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris
| Réactivité | Humain, souris |
| Réactivité citée | Humain |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | IFNAR1 Protéine recombinante Ag3739 |
| Nom complet | interferon (alpha, beta and omega) receptor 1 |
| Masse moléculaire calculée | 557 aa, 64 kDa |
| Poids moléculaire observé | 64 kDa |
| Numéro d’acquisition GenBank | BC021825 |
| Symbole du gène | IFNAR1 |
| Identification du gène (NCBI) | 3454 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Interferon alpha and beta receptor subunit 1 (IFNAR1) is a type I membrane protein that associates with IFNAR2 to form the receptor for type I interferons, including interferons-alpha, -beta, and -lambda. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for IFNAR1 antibody 13083-1-AP | Download protocol |
| FC protocol for IFNAR1 antibody 13083-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Rep Degradation of WTAP blocks antiviral responses by reducing the m 6 A levels of IRF3 and IFNAR1 mRNA | ||
Elife The oncoprotein BCL6 enables solid tumor cells to evade genotoxic stress.
| ||
Cell Rep TDP-43 prevents endogenous RNAs from triggering a lethal RIG-I-dependent interferon response. | ||
J Nat Med Baicalin promotes antiviral IFNs production and alleviates type I IFN-induced neutrophil inflammation |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Christine (Verified Customer) (07-12-2022) | Both cell lines are human ones, only the RPE1 cells express the IFNAR1 receptor. IFNAR1 is described in the literature as heavily glycosylated and hence its molecular weight can vary from 64-135 kDa depending on its glycosylation level. We generally observed in RPE1 cells fully matured 110-130 kDa major band on western blot, which is not observed at all here with the 13083-1-AP antibody. The 72 kDa bands seems to be present only in RPE1 and not in the IFNAR1 null Huh7 cells, indicating it could indeed be immature non glycosylated forms of IFNAR1. (We also observed these minor bands on RPE1 cells with another IFNAR1 antibody , but at a much lower intensity than the 110 kDa one)
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