Anticorps Polyclonal de lapin anti-Rabbit IgG control

Rabbit IgG control Polyclonal Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

n/a

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

N° de cat : CL647-30000

Synonymes

Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control

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Galerie de données de validation

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  FC experiment of HeLa using CL647-30000

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Rabbit IgG control (CL647-30000) (red), or 0.2 ug CoraLite® Plus 647-conjugated TRAP1 Rabbit PolyAb (CL647-10325) (green), or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-30000 cible Rabbit IgG control dans les applications de FC (Intra) et montre une réactivité avec des échantillons n/a

• Réactivité: n/a
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: Rabbit IgG control
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: N/A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. This antibody can be used as Rabbit IgG control in experiments.

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