Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, L02 cells, MCF-7 cells, MDA-MB-231 cells, U-937 cells |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue, human gliomas tissue, human pancreas tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human ovary tumor tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21997-1-AP targets CD107a / LAMP1 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, monkey, zebrafish, bovine, turtle |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17367 Product name: Recombinant human CD107a protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 35-377 aa of BC021288 Sequence: NGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLL Predict reactive species |
| Full Name | lysosomal-associated membrane protein 1 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 90-120 kDa |
| GenBank Accession Number | BC021288 |
| Gene Symbol | LAMP1 |
| Gene ID (NCBI) | 3916 |
| ENSEMBL Gene ID | ENSG00000185896 |
| RRID | AB_2878966 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11279 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD107a / LAMP1 antibody 21997-1-AP | Download protocol |
| IHC protocol for CD107a / LAMP1 antibody 21997-1-AP | Download protocol |
| WB protocol for CD107a / LAMP1 antibody 21997-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD107a / LAMP1 Polyclonal antibody (Cat# 21997-1-AP) has 223 citations. These appear across high-impact journals including Autophagy, Nature Communications, Advanced Science, Cell Metabolism, Immunity, Molecular Cell, Oncogene, and PNAS. Research fields span autophagy and lysosomal function, cancer biology, neurodegeneration (Alzheimer's, Parkinson's), lipid metabolism, ferroptosis, and immunology, with applications mainly in WB, IHC, and IP.
| Species | Application | Title |
|---|---|---|
Cell Metab Oral GLP-1 receptor agonist promotes astrocyte-neuron lactate and lipid transfer with neuroprotective effects. | ||
Immunity The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis. | ||
Bone Res Chaperone-mediated autophagy directs a dual mechanism to balance premature senescence and senolysis to prevent intervertebral disc degeneration | ||
Autophagy Microplastics exacerbate ferroptosis via mitochondrial reactive oxygen species-mediated autophagy in chronic obstructive pulmonary disease | ||
Autophagy Lipotoxic hepatocyte-derived UBQLN1-enriched small extracellular vesicles activate hepatic stellate cells to promote hepatic fibrosis. | ||
Autophagy Influenza A virus protein PB1-F2 impairs innate immunity by inducing mitophagy. |
Reviews
What customers say
All four reviews give 5 stars. Users report reliable LAMP1 detection by Western blot and immunohistochemistry/immunocytochemistry. Western blotting at 1:1000–1:2000 produced clear bands, including in human breast cancer and H4 cells, while immunostaining at 1:50 gave strong labeling in fixed NSC-34 cells. Overall, reviewers describe the antibody as working great for detecting LAMP1 expression.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zakia (Verified Customer) (05-22-2024) | Works great.
|
Marina (Verified Customer) (08-08-2022) | Western blot against LAMP1 in human breast cancer cell line HCC1937. 1:1000 dilution, overnight incubation at 4ºC. A clear band can be observed between 95 and 130 kDa. Ponceau S staining is included as total protein loading control.
 |
Azita (Verified Customer) (06-17-2021) | Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by LAMP1 Polyclonal antibody at dilution of 1:50 showed strong labelling.
|
Mayru (Verified Customer) (04-30-2019) | The antibody works great for detection of LAMP1 protein expression using Western blot analysis.
|
