Anticorps Monoclonal anti-LC3A/B

LC3A/B Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris et plus (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(25)

CloneNo.

2B2B5

N° de cat : 66139-1-Ig

Synonymes

LC3, LC3A, LC3A/B, LC3B, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A, MAP1LC3A/B, MAP1LC3B

---

Galerie de données de validation

• 

---

Applications publiées

• WB: See 19 publications below
• IHC: See 1 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Informations sur le produit

66139-1-Ig cible LC3A/B dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: rat, Humain, porc, souris, Hamster
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: microtubule-associated protein 1 light chain 3 alpha
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14 kDa, 16 kDa
• Numéro d’acquisition GenBank: NM_032514
• Symbole du gène: LC3
• Identification du gène (NCBI): 84557
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II.

Publications

Species	Application	Title
mouse	IF	Cell Res; NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.; Authors - Min Liu
rat	WB	Phytomedicine; Salidroside facilitates neuroprotective effects in ischemic stroke by promoting axonal sprouting through promoting autophagy; Authors - Wenfang Lai
mouse,rat	WB,IP	Cell Death Dis; Exogenous H2S facilitating ubiquitin aggregates clearance via autophagy attenuates type 2 diabetes-induced cardiomyopathy.; Authors - Jichao Wu
pig	IF	Cell Prolif; S-nitrosoglutathione reductase maintains mitochondrial homeostasis by promoting clearance of damaged mitochondria in porcine preimplantation embryos.; Authors - Ying-Jie Niu
mouse	IF	Mol Neurobiol; Colistin-Induced Apoptosis of Neuroblastoma-2a Cells Involves the Generation of Reactive Oxygen Species, Mitochondrial Dysfunction, and Autophagy.; Authors - Chongshan Dai
pig	WB	Aging (Albany NY); Ubiquinol-10 delays postovulatory oocyte aging by improving mitochondrial renewal in pigs.; Authors - Ying-Jie Niu

{{ptg:RelatedPrimaryAntibodies}}