Anticorps Monoclonal anti-MHC Class II (I-A/I-E)

MHC Class II (I-A/I-E) Monoclonal Antibody for FC, IF

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

IF, FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

M5/114.15.2

N° de cat : CL488-65122

Synonymes

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II



Applications testées

Résultats positifs en IFsplénocytes de souris,
Résultats positifs en cytométriesplénocytes de souris,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65122 cible MHC Class II (I-A/I-E) dans les applications de IF, FC et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Rat / IgG2b, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Cellules spléniques activées de souris C57BL/6
Nom complet histocompatibility 2, class II antigen A, alpha
Numéro d’acquisition GenBankBC031711
Symbole du gène H2-Aa
Identification du gène (NCBI) 14960
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 MHC Class II (I-A/I-E) antibody CL488-65122Download protocol
Standard Protocols
Click here to view our Standard Protocols