Anticorps Polyclonal de lapin anti-MRPL37

MRPL37 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 15190-1-AP

Synonymes

L2mt, L37mt, MRP L2, MRP L37, MRPL2, MRPL37, RPML2

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Galerie de données de validation

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  WB analysis of mouse liver using 15190-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15190-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 15190-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 15190-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 15190-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 15190-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15190-1-AP (MRPL37 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 15190-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15190-1-AP (MRPL37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 15190-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15190-1-AP (MRPL37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 15190-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15190-1-AP (MRPL37 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 15190-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15190-1-AP (MRPL37 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 15190-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15190-1-AP (MRPL37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu cardiaque humain, tissu ovarien de souris, tissu utérin de souris
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below

Informations sur le produit

15190-1-AP cible MRPL37 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MRPL37 Protéine recombinante Ag7276
• Nom complet: mitochondrial ribosomal protein L37
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC000041
• Symbole du gène: MRPL37
• Identification du gène (NCBI): 51253
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Sci Adv; Adult-onset obesity is triggered by impaired mitochondrial gene expression.; Authors - Kara L Perks
mouse	WB	Sci Adv; Fidelity of translation initiation is required for coordinated respiratory complex assembly.; Authors - Danielle L Rudler
mouse	WB	EMBO J; Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.; Authors - Nicola Ferreira
mouse	WB	Aging Cell; Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.; Authors - Tara R Richman
human	WB	Am J Hum Genet; Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome.; Authors - Nicole J Lake
mouse	WB	EMBO Rep; Concerted regulation of mitochondrial and nuclear non-coding RNAs by a dual-targeted RNase Z.; Authors - Stefan J Siira