Anticorps Monoclonal anti-MSH2

• Phare
• Validé par KD/KO

MSH2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2H5F5

N° de cat : 60161-1-Ig

Synonymes

2H5F5, MSH 2

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Galerie de données de validation

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  WB analysis of HeLa using 60161-1-Ig

  WB results of MSH2 (60161-1-Ig, 1:1000) antibody with si-Control and si-MSH2 transfected HeLa cells..

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  WB analysis using 60161-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60161-1-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60161-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60161-1-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 60161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Ramos using 60161-1-Ig

  Immunohistochemical analysis of paraffin-embedded Ramos cells slide using 60161-1-Ig (MSH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 60161-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MSH2 antibody (60161-1-Ig, Clone: 2H5F5 ) at dilution of 1:2277 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005).

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  IF Staining of A431 using 60161-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MSH2 antibody (60161-1-Ig, Clone: 2H5F5 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, cellules HCT 116, cellules HEK-293, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du côlon humain, cellules Ramos; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

60161-1-Ig cible MSH2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MSH2 Protéine recombinante Ag7835
• Nom complet: mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC021566
• Symbole du gène: MSH2
• Identification du gène (NCBI): 4436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

Publications

Species	Application	Title
mouse	IHC	Cancer Immunol Res; Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite Stability.; Authors - Qijing Wu
human	WB	Sci Rep; Cold atmospheric plasma stabilizes mismatch repair for effective, uniform treatment of diverse colorectal cancer cell types; Authors - Yuanyuan He
human	WB	Transl Res; Novel MSH2 and TSC2 variants in a Chinese family with Lynch syndrome and their synergistic impact in urothelial carcinoma; Authors - Mingyang Li

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