Anticorps Polyclonal de lapin anti-Acetyl-Histone H3 (Lys9)

• Phare
• Validé par KD/KO

Acetyl-Histone H3 (Lys9) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 29133-1-AP

Synonymes

HIST1H3A, Histone-H3, HistoneH3, Histone H3, Histone H 3

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Galerie de données de validation

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  WB analysis using 29133-1-AP

  Untreated and Trichostatin A treated HeLa cell lysates were subjected to SDS PAGE followed by western blot with 29133-1-AP (Mono-Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 29133-1-AP

  WB result of Mono-Acetyl-Histone H3 (Lys9) antibody (29133-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-Mono-Acetyl-Histone H3 (Lys9) transfected HEK-293 cells.

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Applications testées

• Résultats positifs en WB: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

29133-1-AP cible Acetyl-Histone H3 (Lys9) dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone cluster 1, H3a
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC066245
• Symbole du gène: HIST1H3A
• Identification du gène (NCBI): 8350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.

Publications

Species	Application	Title
rat	IHC	Neurochem Res; The Role of Acetylation and Methylation of Rat Hippocampal Histone H3 in the Mechanism of Aluminum-Induced Neurotoxicity; Authors - Jie Gao
human	WB	Nature; Transcriptional adaptation upregulates utrophin in Duchenne muscular dystrophy; Authors - Lara Falcucci
human,mouse	WB,IF	J Pharmacol Sci; Unravelling the effects of specific ingredients of kamishoyosan in reducing aggressive biting behavior in chronically isolated mice to inanimate objects; Authors - Kento Igarashi

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