Acetyl-Histone H3 (Lys9) Recombinant monoclonal antibody, PBS Only

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  WB analysis of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

  Trichostatin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin Monoclonal antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 84543-1-RR (same clone as 84543-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 84543-1-RR (Acetyl-Histone H3 (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84543-1-RR, Clone: 241596F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 84543-1-RR (same clone as 84543-1-PBS)

  Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys9) (84543-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

• 

  ChIP experiment of HeLa using 84543-1-RR (same clone as 84543-1-PBS)

  Chromatin was prepared from HeLa cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys9) (84543-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84543-1-PBS in a different storage buffer formulation.

Acetyl-Histone H3 (Lys9) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR

Cat No. 84543-1-PBS

Clone No.241596F9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR

Synonyms

Acetyl Histone, H3 Ac Lys9, H3 Acetyl K9, H3 Acetyl Lys9, H3 Acetyl Lysine9

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Product Information

84543-1-PBS targets Acetyl-Histone H3 (Lys9) in WB, IF/ICC, ChIP, Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P68431
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.