Anticorps Monoclonal anti-IgG1 Isotype Control

IgG1 Isotype Control Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Megathura crenulata et plus (1)

Applications

IF, FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(1)

CloneNo.

1F8D3

N° de cat : CL488-66360-1

Synonymes

Mouse IgG1 isotype control, Kappa light chain, Mouse IgG1

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Galerie de données de validation

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  FC experiment of A431 using CL488-66360-1

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Mouse IgG1 isotype control (CL488-66360-1, Clone: 1F8D3) (red), or 0.4 ug CoraLite® Plus 488 Anti-Human FKBP8 (CL488-66690, Clone: 1C1G12) (green), or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of human PBMCs using CL488-66360-1

  1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 488 Anti-IgG1 Isotype Control (CL488-66360-1, Clone:1F8D3) (red), CL488 Anti-Human CD35 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines, cellules A431

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL488-66360-1 cible IgG1 Isotype Control dans les applications de IF, FC et montre une réactivité avec des échantillons Megathura crenulata

• Réactivité: Megathura crenulata
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: IgG1 Isotype Control
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Publications

Species	Application	Title
mouse	IF	Front Immunol; Immunometabolic analysis of primary murine group 2 innate lymphoid cells: a robust step-by-step approach; Authors - Sai Sakktee Krisna

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