Anticorps Monoclonal anti-Nephrin

Nephrin Monoclonal Antibody for IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(16)

CloneNo.

1D11H8

N° de cat : 66970-1-Ig

Synonymes

NPHS1, 1D11H8, CNF, NPHN, NPHS 1

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Galerie de données de validation

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  IHC staining of human kidney using 66970-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66970-1-Ig (Nephrin antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66970-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66970-1-Ig (Nephrin antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 10 publications below

Informations sur le produit

66970-1-Ig cible Nephrin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Nephrin Protéine recombinante Ag19273
• Nom complet: nephrosis 1, congenital, Finnish type (nephrin)
• Masse moléculaire calculée: 1241 aa, 135 kDa
• Numéro d’acquisition GenBank: BC156935
• Symbole du gène: Nephrin
• Identification du gène (NCBI): 4868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).

Publications

Species	Application	Title
	WB	Phytomedicine; Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro; Authors - Zhongzhu Ai
	WB,IF	Phytomedicine; Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis; Authors - Daofei Song
	IF	Genomics; Effectiveness of a novel rat model of off-target PLA2R1 knockout to renal impairment; Authors - Bo Huang
human	WB	Mol Cell Endocrinol; Experimental study on renoprotective effect of intermedin on diabetic nephropathy.; Authors - Yanhong Wang
	WB	Exp Mol Pathol; Urinary exosomes from patients with diabetic kidney disease induced podocyte apoptosis via microRNA-145-5p/Srgap2 and the RhoA/ROCK pathway; Authors - Lulu Han
	WB,IHC,IF	Heliyon; QiDiTangShen granules alleviates diabetic nephropathy podocyte injury: A network pharmacology study and experimental validation in vivo and vitro; Authors - Fei Gao

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