Tested Applications
| Positive WB detected in | MOLT-4 cells, HepG2 cells, HEK-293 cells |
| Positive IHC detected in | human cervical squamous cancer tissue, human ovary cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human cervical cancer tissue |
| Positive FC (Intra) detected in | U-937 cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83341-1-RR targets Osteopontin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0754 Product name: Recombinant human OPN, SPP1 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 17-300 aa of BC007016 Sequence: IPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQTLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN Predict reactive species |
| Full Name | secreted phosphoprotein 1 |
| Calculated Molecular Weight | 314 aa, 35 kDa |
| Observed Molecular Weight | 60-66 kDa |
| GenBank Accession Number | BC007016 |
| Gene Symbol | Osteopontin |
| Gene ID (NCBI) | 6696 |
| RRID | AB_3671001 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P10451-5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands corresponding to peptide fragments (PMID: 8195113; 17890765).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Osteopontin antibody 83341-1-RR | Download protocol |
| IF protocol for Osteopontin antibody 83341-1-RR | Download protocol |
| IHC protocol for Osteopontin antibody 83341-1-RR | Download protocol |
| WB protocol for Osteopontin antibody 83341-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
83341-1-RR is an anti-SPP1 (osteopontin) rabbit recombinant antibody with 20 citations. Publications appear in journals including Gut, Nature Communications, Advanced Science, Oncogene, EMBO Molecular Medicine, Molecular Metabolism, Journal of Gastroenterology, and iScience. Research fields span hepatocellular carcinoma, prostate cancer, head and neck cancer, breast cancer, pancreatic cancer, lung cancer, aortic dissection, aortic aneurysm, osteoporosis, liver cirrhosis, muscle atrophy, bone regeneration, traumatic brain injury, and aortic valve calcification.
| Species | Application | Title |
|---|---|---|
Gut CD48 is a novel immune checkpoint on tumour-associated macrophages in hepatocellular carcinoma | ||
Nat Commun Improving erectile function in diabetic male mice by rescuing depalmitoylated FBP1 to reduce cavernosal lactate. | ||
Adv Sci (Weinh) Light-Switched Mesenchymal Stem Cells for In Situ Exosome Amplification in Craniofacial Bone Defect Reconstruction. | ||
Oncogene PKA-driven SPP1 activation as a novel mechanism connecting the bone microenvironment to prostate cancer progression | ||
Arterioscler Thromb Vasc Biol Exercise Attenuates Aortic Dissection Via PDE5A-Mediated Inhibition of Vascular Smooth Muscle Cell Phenotypic Switch. | ||
EMBO Mol Med Brown remodeling of white adipose tissue protects against abdominal aortic aneurysm via batokine FSTL1. |
Reviews
What customers say
The antibody performed excellently for IHC staining in human PDX models growing subcutaneously and in mouse bone. The reviewer reported highly specific and strong staining in positive samples, using a 1:200 dilution. Overall, the product received a 5-star rating, reflecting satisfaction with its performance in immunohistochemistry applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Pablo (Verified Customer) (10-15-2025) | The antibody worked perfectly for IHC staining in human PDX growing subcutaneously as well as in mice bone. The staining was specific and very strong in positive samples.
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