Anticorps Polyclonal de lapin anti-PFKFB2

PFKFB2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 17838-1-AP

Synonymes

Fructose-2,6-bisphosphatase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, 6-phosphofructo-2-kinase, 6PF-2-K/Fru-2,6-P2ase heart-type isozyme, 6PF-2-K/Fru-2,6-P2ase 2

---

Galerie de données de validation

• 

  WB analysis using 17838-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17838-1-AP (PFKFB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse retina using 17838-1-AP

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 17838-1-AP (PFKFB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules LNCaP, tissu rétinien de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

17838-1-AP cible PFKFB2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PFKFB2 Protéine recombinante Ag12181
• Nom complet: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
• Masse moléculaire calculée: 505 aa, 58 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC075076
• Symbole du gène: PFKFB2
• Identification du gène (NCBI): 5208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PFKFB2(6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatase 2) is also named as PFK-2/FBPase-2, which is a bifunctional enzyme responsible for the synthesis and breakdown of Fru-2,6-P2 in the regulation of glycolysis(PMID:10095107). In the C-terminal section, it belongs to the phosphoglycerate mutase family. The enzyme is a dimer of identical subunits, each composed of 505 amino acids and containing three discrete domains(PMID: 2552438). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
rat	WB	Elife; Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control; Authors - Meiling Wu
human	WB	Cell Death Dis; SLIT2/ROBO1 axis contributes to the Warburg effect in osteosarcoma through activation of SRC/ERK/c-MYC/PFKFB2 pathway.; Authors - Shu-Jie Zhao
human	WB	Free Radic Biol Med; Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.; Authors - Kelei Dong
mouse	WB	J Biol Chem; Type 2 Diabetes Dysregulates Glucose Metabolism in Cardiac Progenitor Cells.; Authors - Joshua K Salabei
human	WB	Int J Biochem Cell Biol; A transcriptional target of androgen receptor, miR-421 regulates proliferation and metabolism of prostate cancer cells.; Authors - Delong Meng
human	WB	J Transl Med; Small molecule inhibition of 6-phosphofructo-2-kinase suppresses t cell activation.; Authors - Telang Sucheta S

{{ptg:RelatedPrimaryAntibodies}}