Anticorps Polyclonal de lapin anti-PTEN

• Phare
• Validé par KD/KO

PTEN Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 10047-1-AP

Synonymes

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

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Galerie de données de validation

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  WB analysis of HeLa using 10047-1-AP

  WB result of PTEN antibody (10047-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTEN transfected HeLa cells.

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  WB analysis using 10047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10047-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10047-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 10047-1-AP

  IP result of anti-PTEN(IP:10047-1-AP, 4ug; Detection:10047-1-AP 1:3000) with HEK-293T cells lysate 2040 ug.

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  IHC staining of human breast cancer using 10047-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10047-1-AP (PTEN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10047-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10047-1-AP (PTEN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules DU 145, cellules HeLa
• Résultats positifs en IP: cellules HEK-293T,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

10047-1-AP cible PTEN dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTEN Protéine recombinante Ag0007
• Nom complet: phosphatase and tensin homolog
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC005821
• Symbole du gène: PTEN
• Identification du gène (NCBI): 5728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Oxid Med Cell Longev; Knockdown of miR-214 Alleviates Renal Interstitial Fibrosis by Targeting the Regulation of the PTEN/PI3K/AKT Signalling Pathway; Authors - DongHua Hou
human	WB	Redox Biol; Impairment of Akt activity by CYP2E1 mediated oxidative stress is involved in chronic ethanol-induced fatty liver.; Authors - Tao Zeng
rat	WB,IHC	Cancers (Basel); Astragaloside IV Inhibits the Proliferation of Human Uterine Leiomyomas by Targeting IDO1; Authors - Tiantian Qiu
human	WB	J Cell Mol Med; Knockdown of CENPK inhibits cell growth and facilitates apoptosis via PTEN-PI3K-AKT signalling pathway in gastric cancer.; Authors - Shusheng Wu
human,mouse	WB,IF	iScience; Smurf1 silencing restores PTEN expression that ameliorates progression of human glioblastoma and sensitizes tumor cells to mTORC1/C2 inhibitor Torin1.; Authors - Qin Xia ; KD Validated
human	WB,IHC	J Biomol Struct Dyn; Molecular dynamic simulation and functional analysis of pathogenic PTEN mutations in glioblastoma; Authors - Vildan Bozok Cetintas