Anticorps Monoclonal anti-PTEN

• Phare
• Validé par KD/KO

PTEN Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

34

CloneNo.

5C10B6

Avis (1)

N° de cat : 60300-1-Ig

Synonymes

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

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Galerie de données de validation

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  WB analysis using 60300-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60300-1-Ig (PTEN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 60300-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60300-1-Ig (PTEN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 60300-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60300-1-Ig (PTEN Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast hyperplasia using 60300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60300-1-Ig (PTEN antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60300-1-Ig (PTEN antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60300-1-Ig (PTEN Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60300-1-Ig (PTEN Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 60300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using (PTEN Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 60300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using (PTEN Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 60300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60300-1-Ig (PTEN antibody) at dilution of 1:50 (under 10x lens) microwave oven heating mediated antigen retrieved with .

• 

  IHC staining of human breast cancer using 60300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60300-1-Ig (PTEN antibody) at dilution of 1:50 (under 40x lens) microwave oven heating mediated antigen retrieved with .

• 

  IHC staining of human lung cancer using 60300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60300-1-Ig (PTEN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60300-1-Ig (PTEN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 60300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60300-1-Ig (PTEN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 60300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60300-1-Ig (PTEN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60300-1-Ig (PTEN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 60300-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60300-1-Ig (PTEN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60300-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PTEN antibody (60300-1-Ig, Clone: 5C10B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7, cellules NIH/3T3, tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu d'hyperplasie mammaire humain, tissu de cancer de l'endomètre humain, tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu d'hyperplasie de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:200-1:1200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 7 publications below
• IF: See 4 publications below

Informations sur le produit

60300-1-Ig cible PTEN dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PTEN Protéine recombinante Ag17274
• Nom complet: phosphatase and tensin homolog
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC005821
• Symbole du gène: PTEN
• Identification du gène (NCBI): 5728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.

Publications

Species	Application	Title
human	WB	Mol Cell; Fumarate inhibits PTEN to promote tumorigenesis and therapeutic resistance of type2 papillary renal cell carcinoma.; Authors - Xin Ge
human	IHC	Hepatology; Long noncoding RNA TSLNC8 is a tumor suppressor that inactivates the interleukin-6/STAT3 signaling pathway.; Authors - Jiwei Zhang
human	WB	Hepatology; Cyclin G1-mediated epithelial-mesenchymal transition via phosphoinositide 3-kinase/Akt signaling facilitates liver cancer progression.; Authors - Wen Wen W
human	IF	Nat Commun; The deubiquitylase OTUD3 stabilizes GRP78 and promotes lung tumorigenesis.; Authors - Tongde Du
mouse	IHC	Cancer Res; Targeting USP7 identifies a metastasis-competent state within bone marrow-resident melanoma CTCs.; Authors - Monika Vishnoi
human	WB	J Transl Med; HNRNPA2B1-mediated m6A modification of lncRNA MEG3 facilitates tumorigenesis and metastasis of non-small cell lung cancer by regulating miR-21-5p/PTEN axis; Authors - Ke Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Georgina (Verified Customer) (11-04-2021)

Good primary antibody for PTEN. There is some background staining, however the band for PTEN is clear.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000

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