Phospho-LAT (Tyr220) Recombinant monoclonal antibody, PBS Only

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• Phospho-LAT (Tyr220) Recombinant monoclonal antibody, PBS Only (87289-1-PBS)

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  WB analysis of Jurkat using 87289-1-RR (same clone as 87289-1-PBS)

  Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 87289-1-RR (Phospho-LAT (Tyr220) antibody) blocked with BSA only, Phospho-LAT (Tyr220) peptide or non-Phospho peptide were subjected to SDS PAGE followed by western blot with 87289-1-RR (LAT-Tyr220 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87289-1-PBS in a different storage buffer formulation.

Phospho-LAT (Tyr220) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87289-1-PBS

Clone No.252580D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

LAT (Tyr220), Phospho LAT (Tyr220), Phospho-LAT, p-LAT (Tyr220), p-LAT (Y220)

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Product Information

87289-1-PBS targets Phospho-LAT (Tyr220) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: linker for activation of T cells
• Calculated Molecular Weight: 262 aa, 28 kDa
• Observed Molecular Weight: 36 kDa, 38 kDa
• GenBank Accession Number: BC011563
• Gene Symbol: LAT
• Gene ID (NCBI): 27040
• RRID: AB_3745449
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43561
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LAT is a membrane-localized adaptor protein which is primarily concentrated in microdomains by palmitoylation. Phospho-LAT (Tyr220) is a phosphorylated form of the Linker for Activation of T cells (LAT), a protein that plays a pivotal role in T cell receptor (TCR) signaling. LAT is involved in the assembly of the signaling complex that leads to T cell activation. Upon TCR engagement, LAT becomes phosphorylated at multiple tyrosine residues, including Tyr220. (PMID: 38746248)