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Tested Applications
| Positive WB detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87289-1-RR targets Phospho-LAT (Tyr220) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | linker for activation of T cells |
| Calculated Molecular Weight | 262 aa, 28 kDa |
| Observed Molecular Weight | 36 kDa, 38 kDa |
| GenBank Accession Number | BC011563 |
| Gene Symbol | LAT |
| Gene ID (NCBI) | 27040 |
| RRID | AB_3745449 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O43561 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LAT is a membrane-localized adaptor protein which is primarily concentrated in microdomains by palmitoylation. Phospho-LAT (Tyr220) is a phosphorylated form of the Linker for Activation of T cells (LAT), a protein that plays a pivotal role in T cell receptor (TCR) signaling. LAT is involved in the assembly of the signaling complex that leads to T cell activation. Upon TCR engagement, LAT becomes phosphorylated at multiple tyrosine residues, including Tyr220. (PMID: 38746248)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-LAT (Tyr220) antibody 87289-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
