Anticorps Polyclonal de lapin anti-RB1CC1

• Phare
• Validé par KD/KO

RB1CC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(21)

N° de cat : 10043-2-AP

Synonymes

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa

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Galerie de données de validation

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  WB analysis of HeLa using 10043-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10043-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10043-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10043-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10043-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10043-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10043-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10043-2-AP (RB1CC1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10043-2-AP

  Immunofluorescent analysis of Hela cells, using RB1CC1 antibody 10043-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules MCF-7, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

10043-2-AP cible RB1CC1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: RB1-inducible coiled-coil 1
• Masse moléculaire calculée: 1594 aa, 183 kDa
• Poids moléculaire observé: 180-200 kDa
• Numéro d’acquisition GenBank: BC017556
• Symbole du gène: RB1CC1
• Identification du gène (NCBI): 9821
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation[PMID:15968549 ]. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation [PMID: 19258318]. It is probably involved in the tumorigenesis of breast cancer[PMID: 14533007]. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene[PMID: 21602932]. The immunogen of this antibody is C-terminal 154aa of RB1CC1.

Publications

Species	Application	Title
mouse	WB	Cell; Noncanonical autophagy promotes the visual cycle.; Authors - Kim Ji-Young JY ; KD Validated
human	WB	Nat Immunol; Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms.; Authors - Michal P Wandel ; KD Validated
human	WB	Nat Cell Biol; mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6.; Authors - Nazio Francesca F
human	IF	Nat Cell Biol; ATM functions at the peroxisome to induce pexophagy in response to ROS.; Authors - Jiangwei Zhang
mouse	WB	Genes Dev; p62/SQSTM1 synergizes with autophagy for tumor growth in vivo.; Authors - Huijun Wei
human, mouse	WB, IF	Nat Struct Mol Biol; Interaction between FIP200 and ATG16L1 distinguishes ULK1 complex-dependent and -independent autophagy.; Authors - Noor Gammoh

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