Anticorps Monoclonal anti-TRAF6

• Phare
• Validé par KD/KO

TRAF6 Monoclonal Antibody for FC, IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

55

CloneNo.

1D1E1

Avis (2)

N° de cat : 66498-1-Ig

Synonymes

RING finger protein 85, RNF85, TRAF6

---

Galerie de données de validation

• 

  WB analysis using 66498-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66498-1-Ig (TRAF6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66498-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66498-1-Ig (TRAF6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66498-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66498-1-Ig (TRAF6 antibody) at dilution of 1:300 (under 10x lens).

• 

  IHC staining of human liver using 66498-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66498-1-Ig (TRAF6 antibody) at dilution of 1:300 (under 40x lens).

• 

  IHC staining of human heart using 66498-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66498-1-Ig (TRAF6 antibody) at dilution of 1:300 (under 10x lens).

• 

  IHC staining of human heart using 66498-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66498-1-Ig (TRAF6 antibody) at dilution of 1:300 (under 40x lens).

• 

  IF Staining of HeLa using 66498-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF6 antibody (66498-1-Ig, Clone: 1D1E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Jurkat using 66498-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human TRAF6 (66498-1-Ig, Clone:1D1E1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules U2OS
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 50 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 3 publications below

Informations sur le produit

66498-1-Ig cible TRAF6 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, fish
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TRAF6 Protéine recombinante Ag24696
• Nom complet: TNF receptor-associated factor 6
• Masse moléculaire calculée: 522 aa, 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC031052
• Symbole du gène: TRAF6
• Identification du gène (NCBI): 7189
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.

Publications

Species	Application	Title
human	WB,IF	Autophagy; Increased alveolar epithelial TRAF6 via autophagy-dependent TRIM37 degradation mediates particulate matter-induced lung metastasis; Authors - Jiajun Liu
human	WB	EBioMedicine; PMID: 31594754; Authors - Yiyi Liu
human	WB	Cell Death Differ; Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling; Authors - Shunfeng Hu
human	IHC	J Immunother Cancer; Helicobacter pylori infection disturbs the tumor immune microenvironment and is associated with a discrepant prognosis in gastric de novo diffuse large B-cell lymphoma.; Authors - Yuwei Deng
human	WB	Br J Pharmacol; Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A; Authors - Lu Zhang
	WB	Theranostics; Fibrinogen-like protein 2 aggravates nonalcoholic steatohepatitis via interaction with TLR4, eliciting inflammation in macrophages and inducing hepatic lipid metabolism disorder.; Authors - Junjian Hu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

Andrea (Verified Customer) (12-07-2018)

Antibody works well for western blot at a dilution of 1:8000. Did not work for immunoprecipitation.

• Applications: Western Blot, Immunoprecipitation,

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C

TRAF6 Polyclonal antibody

12809-1-AP

IRAK1 Polyclonal antibody

10478-2-AP

TANK Polyclonal antibody

27065-1-AP

TAK1 Polyclonal antibody

12330-2-AP

NFKB1,p105,p50 Polyclonal antibody

14220-1-AP

MYD88 Polyclonal antibody

23230-1-AP

TRAF3 Polyclonal antibody

18099-1-AP