Anticorps Polyclonal de lapin anti-TRPV1

TRPV1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 22686-1-AP

Synonymes

Osm 9 like TRP channel 1, Osm-9-like TRP channel 1, OTRPC1, Vanilloid receptor 1, VR1

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Galerie de données de validation

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  WB analysis of PC-12 using 22686-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 22686-1-AP (TRPV1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 22686-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22686-1-AP (TRPV1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 22686-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22686-1-AP (TRPV1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse skin using 22686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 22686-1-AP (TRPV1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse skin using 22686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 22686-1-AP (TRPV1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 22686-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 22686-1-AP (TRPV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 22686-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 22686-1-AP (TRPV1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 22686-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TRPV1 antibody (22686-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-12
• Résultats positifs en IHC: tissu cutané humain, tissu cutané de souris, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

22686-1-AP cible TRPV1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRPV1 Protéine recombinante Ag18583
• Nom complet: transient receptor potential cation channel, subfamily V, member 1
• Masse moléculaire calculée: 839 aa, 95 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC132820
• Symbole du gène: TRPV1
• Identification du gène (NCBI): 7442
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRPV1, also named as VR1 or OTRPC1, belongs to the transient receptor potential (TRP) family and TRPV subfamily (PMID: 17579562). TRPV1 is a ligand-activated non-selective calcium permeant cation channel expressed predominantly by sensory neurons (PMID: 10821274). It is involved in detection of noxious chemical and thermal stimuli. TRPV1 plays an important role in inflammatory thermal hyperalgesia (PMID: 10764638; 10821274).

Publications

Species	Application	Title
human	WB	Acta Pharmacol Sin; PMID: 29188798; Authors - Su-Shan Zhang
mouse	WB	J Neurosci; Phosphorylation of TRPV1 S801 Contributes to Modality-Specific Hyperalgesia in Mice.; Authors - John Joseph
mouse	IHC	Oncotarget; Dietary capsaicin and antibiotics act synergistically to reduce non-alcoholic fatty liver disease induced by high fat diet in mice.; Authors - Jingjuan Hu
rat	WB	Biomed Pharmacother; Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma.; Authors - Weiwei Qin
mouse	WB	Biomed Pharmacother; Qingfei oral liquid alleviates airway hyperresponsiveness and mucus hypersecretion via TRPV1 signaling in RSV-infected asthmatic mice.; Authors - Xiaoping Jing
mouse	WB	Eur J Pharmacol; Spinophilin negatively controlled the function of transient receptor potential vanilloid 1 in dorsal root ganglia neurons of mice.; Authors - Zhen Guo

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