Tested Applications
| Positive WB detected in | PC-3 cells, Daudi cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10444-1-AP targets SLC43A1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0305 Product name: Recombinant human SLC43A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 107-320 aa of BC001639 Sequence: RLVGSACFTASCTLMALASRDVEALSPLIFLALSLNGFGGICLTFTSLTLPNMFGNLRSTLMALMIGSYASSAITFPGIKLIYDAGVAFVVIMFTWSGLACLIFLNCTLNWPIEAFPAPEEVNYTKKIKLSGLALDHKVTGDLFYTHVTTMGQRLSQKAPSLEDGSDAFMSPQDVRGTSENLPERSVPLRKSLCSPTFLWSLLTMGMTQLRIIF Predict reactive species |
| Full Name | solute carrier family 43, member 1 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 55-61 kDa |
| GenBank Accession Number | BC001639 |
| Gene Symbol | SLC43A1 |
| Gene ID (NCBI) | 8501 |
| RRID | AB_2302076 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75387 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC43A1, also named as LAT3, PB39 and POV1, belongs to the system L family of plasma membrane carrier proteins that transports large neutral amino acids. It plays a role in the development of human prostate cancer, from prostatic intraepithelial neoplasia to invasive prostate cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLC43A1 antibody 10444-1-AP | Download protocol |
| WB protocol for SLC43A1 antibody 10444-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SLC43A1 Polyclonal antibody (Cat# 10444-1-AP) has 3 total citations. These appear in Nature Communications, Archives of Biochemistry and Biophysics, and Nan Fang Yi Ke Da Xue Xue Bao. The cited research spans hepatocellular carcinoma metabolism, lung adenocarcinoma chemoresistance via amino acid transport barriers, and prenatal stress effects on placental amino acid transport in rats. All three studies used the antibody for Western blot.
| Species | Application | Title |
|---|---|---|
Nat Commun Targeting tRNA-dependent tyrosine usage unveils a metabolic vulnerability in hepatocellular carcinoma. | ||
Arch Biochem Biophys Enhancement of chemoresistance by claudin-1-mediated formation of amino acid barriers in human lung adenocarcinoma A549 cells | ||
Nan Fang Yi Ke Da Xue Xue Bao [Prenatal fear stress impairs cognitive development in offspring rats by disrupting placental amino acid transport]. |
