Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10606-1-AP targets CSNK2A2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0877 Product name: Recombinant human CSNK2A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 50-350 aa of BC008812 Sequence: KYSEVFEAINITNNERVVVKILKPVKKKKIKREVKILENLRGGTNIIKLIDTVKDPVSKTPALVFEYINNTDFKQLYQILTDFDIRFYMYELLKALDYCHSKGIMHRDVKPHNVMIDHQQKKLRLIDWGLAEFYHPAQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRREPFFHGQDNYDQLVRIAKVLGTEELYGYLKKYHIDLDPHFNDILGQHSRKRWENFIHSENRHLVSPEALDLLDKLLRYDHQQRLTAKEAMEHPYFYPVVKEQSQPCADNAVLSSGLTAAR Predict reactive species |
| Full Name | casein kinase 2, alpha prime polypeptide |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC008812 |
| Gene Symbol | CSNK2A2 |
| Gene ID (NCBI) | 1459 |
| RRID | AB_2292447 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P19784 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CSNK2A2(Casein kinase II subunit alpha') is also named as CK2A3, CK2A2, CSNK2A1, CK2 and belongs to the protein kinase superfamily. It can function as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage during mitosis. It also can protect against ionizing radiation-induced apoptosis partly by phosphorylating and activating SIRT1. This protein has molecular weights between 35 kDa and 44 kDa(PMID:8622692).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CSNK2A2 antibody 10606-1-AP | Download protocol |
| IP protocol for CSNK2A2 antibody 10606-1-AP | Download protocol |
| WB protocol for CSNK2A2 antibody 10606-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CSNK2A2 (CK2α') Polyclonal antibody (Cat# 10606-1-AP) has 7 citations published in Nature Communications, Cell Genomics, Cell Reports, Acta Neuropathologica Communications, International Immunopharmacology, Biochemical and Biophysical Research Communications, and bioRxiv. Research fields span neuroinflammation and neurodegeneration, hepatocellular carcinoma, Huntington's disease, ischemic stroke, and embryonic stem cell biology. Applications cited include WB, IHC, IF, and IP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun CK2 inhibition suppresses glial inflammation in models of neuroinflammation and neurodegeneration. | ||
Cell Genom NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors
| ||
Cell Rep Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease
| ||
Acta Neuropathol Commun CK2 alpha prime and alpha-synuclein pathogenic functional interaction mediates synaptic dysregulation in huntington's disease. | ||
Int Immunopharmacol A quinolinyl resveratrol derivative alleviates acute ischemic stroke injury by promoting mitophagy for neuroprotection via targeting CK2α' | ||
Biochem Biophys Res Commun β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells. |
