Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, MDA-MB-453s cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, rat kidney tissue, human stomach cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10695-1-AP targets MPHOSPH6 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1080 Product name: Recombinant human MPHOSPH6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-160 aa of BC005242 Sequence: MAAERKTKLSKNLLRMKFMQRGLDSETKKQLEEEEKKIISEEHWYLDLPELKEKESFIIEEQSFLLCEDLLYGRMSFRGFNPEVEKLMLQMNAKHKAEEVEDETVELDVSDEEMARRYETLVGTIGKKFARKRDHANYEEDENGDITPIKAKKMFLKPQD Predict reactive species |
| Full Name | M-phase phosphoprotein 6 |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 19 kDa |
| GenBank Accession Number | BC005242 |
| Gene Symbol | MPHOSPH6 |
| Gene ID (NCBI) | 10200 |
| RRID | AB_2282161 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99547 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MPHOSPH6 antibody 10695-1-AP | Download protocol |
| IP protocol for MPHOSPH6 antibody 10695-1-AP | Download protocol |
| WB protocol for MPHOSPH6 antibody 10695-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
Genome Med Genome-wide meta-analysis across East Asian and European populations provides insights into the molecular basis of lung cancer. | ||
Cell Prolif The 5.8S pre-rRNA maturation factor, M-phase phosphoprotein 6, is a female fertility factor required for oocyte quality and meiosis. |
Reviews
What customers say
Both reviewers rated the antibody 5 stars for Western Blot at a 1:1000 dilution. One user reported that it specifically recognizes Mphosph6 in mouse embryonic stem cells. The other noted that when 10 µg of total mouse protein was loaded, the antibody also stained the ladder slightly. Overall, users found the antibody effective and specific for its target in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tsimafei (Verified Customer) (12-17-2023) | Nice antibody that specifically recognise Mphosph6
 |
Ralf (Verified Customer) (08-04-2022) | 10µg of the total mouse protein were loaded on the gel. Antibodies also stain the ladder a bit.
 |
