Tested Applications
| Positive WB detected in | HepG2 cells, mouse kidney tissue, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10952-1-AP targets CTDSP1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1410 Product name: Recombinant human CTDSP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC012977 Sequence: MDSSAVITQISKEEARGPLRGKGDQKSAASQKPRSRGILHSLFCCVCRDDGEALPAHSGAPLLVEENGAIPKQTPVQYLLPEAKAQDSDKICVVIDLDETLVHSSFKPVNNADFIIPVEIDGVVHQVYVLKRPHVDEFLQRMGELFECVLFTASLAKYADPVADLLDKWGAFRARLFRESCVFHRGNYVKDLSRLGRDLRRVLILDNSPASYVFHPDNAVPVASWFDNMSDTELHDLLPFFEQLSRVDDVYSVLRQPRPGS Predict reactive species |
| Full Name | CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 29-33 kDa |
| GenBank Accession Number | BC012977 |
| Gene Symbol | CTDSP1 |
| Gene ID (NCBI) | 58190 |
| RRID | AB_2087073 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZU7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CTDSP1 also named as NIF3, NLIIF, or SCP1, is a 261 amino acid protein, which contains one FCP1 homology domain. CTDSP1 localizes in the nucleus and colocalizes with RNA polymerase II. CTDSP1 is expressed in non-neuronal tissues. It is highly expressed in skeletal muscle, spleen, lung and placenta. CTDSP1 preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CTDSP1 antibody 10952-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CTDSP1 Polyclonal antibody (Cat# 10952-1-AP) has 4 citations published in Cell Death Dis, Cell Mol Neurobiol, J Biol Chem, and Iran J Basic Med Sci. Research fields include oral lichen planus and microRNA regulation, neuroprotection in ischemic stroke, neuronal gene regulation via REST, and esophageal squamous cell carcinoma. All citations used the antibody for Western blot in human or mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Mol Neurobiol Elabela, a Novel Peptide, Exerts Neuroprotective Effects Against Ischemic Stroke Through the APJ/miR-124-3p/CTDSP1/AKT Pathway | ||
J Biol Chem Phosphatase activity of Small C-terminal domain phosphatase 1 (SCP1) controls the stability of the key neuronal regulator RE1-silencing transcription factor (REST).
| ||
Iran J Basic Med Sci MiR-574-5p promotes cell proliferation by negatively regulating small C-terminal domain phosphatase 1 in esophageal squamous cell carcinoma |
