Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, Jurkat cells, moust testis |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human pancreas cancer tissue, rat stomach tissue, human rectal cancer tissue, mouse colon tissue, rat brain tissue, rat colon tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11090-1-AP targets DNAJC7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1572 Product name: Recombinant human DNAJC7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 145-494 aa of BC011837 Sequence: YEKIAETDFEKRDFRKVVFCMDRALEFAPACHRFKILKAECLAMLGRYPEAQSVASDILRMDSTNADALYVRGLCLYYEDCIEKAVQFFVQALRMAPDHEKACIACRNAKALKAKKEDGNKAFKEGNYKLAYELYTEALGIDPNNIKTNAKLYCNRGTVNSKLRKLDDAIEDCTNAVKLDDTYIKAYLRRAQCYMDTEQYEEAVRDYEKVYQTEKTKEHKQLLKNAQLELKKSKRKDYYKILGVDKNASEDEIKKAYRKRALMHHPDRHSGASAEVQKEEEKKFKEVGEAFTILSDPKKKTRYDSGQDLDEEGMNMGDFDPNNIFKAFFGGPGGFSFEASGPGNFFFQFG Predict reactive species |
| Full Name | DnaJ (Hsp40) homolog, subfamily C, member 7 |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC011837 |
| Gene Symbol | DNAJC7 |
| Gene ID (NCBI) | 7266 |
| RRID | AB_2293179 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99615 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DnaJC7, otherwise referred to as Tpr2 or CCRP, is a conserved, type of J protein that is highly expressed within the brain. DnaJC7 has been found to modulate the flow of substrates from Hsp70 to Hsp90, providing a control mechanism in proteins such as glucocorticoids and progesterone receptor.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAJC7 antibody 11090-1-AP | Download protocol |
| IHC protocol for DNAJC7 antibody 11090-1-AP | Download protocol |
| IP protocol for DNAJC7 antibody 11090-1-AP | Download protocol |
| WB protocol for DNAJC7 antibody 11090-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-DnaJC7 antibody (Cat# 11090-1-AP) has 12 total citations. These appear in journals including Nature Communications, EMBO Journal, Journal of Biological Chemistry, eLife, Neurobiology of Disease, Biochemical Pharmacology, Cells, Cancers, Life Science Alliance, and Biochemical and Biophysical Research Communications. Research fields span tau/amyloid biology, p53 stabilization, polyglutamine protein aggregation, ovarian cancer, ALS/neurodegeneration, and chaperone-cochaperone interactions.
| Species | Application | Title |
|---|---|---|
Nat Commun DnaJC7 binds natively folded structural elements in tau to inhibit amyloid formation. | ||
EMBO J Microcephaly-associated protein WDR62 supports purine metabolism by interacting with co-chaperone BAG2. | ||
Biochem Pharmacol Indirect activation of constitutive androstane receptor in three-dimensionally cultured HepG2 cells.
| ||
Cells The USP19-DnaJC7 Axis Stabilizes p53 in Cisplatin-Treated Epithelial Ovarian Cancer.
| ||
Neurobiol Dis MAPK family members differentially regulate pThr175 tau-mediated pathogenicity. | ||
Cancers (Basel) Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin |
