Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11275-1-AP targets CREB3 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1823 Product name: Recombinant human CREB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-371 aa of BC009402 Sequence: MELELDAGDQDLLAFLLEESGDLGTAPDEAVRAPLDWALPLSEVPSDWEVDDLLCSLLSPPASLNILSSSNPCLVHHDHTYSLPRETVSMDLESESCRKEGTQMTPQHMEELAEQEIARLVLTDEEKSLLEKEGLILPETLPLTKTEEQILKRVRRKIRNKRSAQESRRKKKVYVGGLESRVLKYTAQNMELQNKVQLLEEQNLSLLDQLRKLQAMVIEISNKTSSSSTCILVLLVSFCLLLVPAIYSSDTRGSLPAEHGVLSRQLRALPSEDPYQLELPALQSEVPKDSTHQWLDGSDCVLQAPGNTSCLLHYMPQAPSAEPPLEWPFPDLFSEPLCRGPILPLQANLTRKGGWLPTGSPSVILQDRYSG Predict reactive species |
| Full Name | cAMP responsive element binding protein 3 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 40-64 kDa |
| GenBank Accession Number | BC009402 |
| Gene Symbol | CREB3 |
| Gene ID (NCBI) | 10488 |
| RRID | AB_10640533 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43889 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CREB3 antibody 11275-1-AP | Download protocol |
| IHC protocol for CREB3 antibody 11275-1-AP | Download protocol |
| WB protocol for CREB3 antibody 11275-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-CREB3 antibody (Cat# 11275-1-AP) has 25 citations spanning journals including Nature Communications, Science Advances, Experimental Molecular Medicine, Free Radical Biology and Medicine, Carcinogenesis, FEBS Letters, Cell Molecular Life Science, International Journal of Molecular Sciences, Translational Oncology, Cancers, Viruses, Molecular Biology Reports, Frontiers in Molecular Neuroscience, and others. Research fields cover neurodegeneration (ALS), Golgi/ER stress, cancer biology (colorectal, hepatocellular, prostate, glioma), metabolic reprogramming, autophagy, ferroptosis, drug resistance, viral infection, and lipid metabolism.
| Species | Application | Title |
|---|---|---|
Nat Commun Golgi stress induces SIRT2 to counteract Shigella infection via defatty-acylation. | ||
Exp Mol Med Dysregulated CREB3 cleavage at the nuclear membrane induces karyoptosis-mediated cell death | ||
Sci Adv Standalone or combinatorial phenylbutyrate therapy shows excellent antiviral activity and mimics CREB3 silencing.
| ||
Free Radic Biol Med Heme oxygenase-1 attenuates sepsis-associated acute lung injury by suppressing the CREB3/ARF4 signaling pathway to mitigate Golgi stress in macrophages.
| ||
Sci Signal High glucose impairs cognitive function through Creb3 O-GlcNAcylation and increased lactate production. |
