EIF2B4 Polyclonal antibody

EIF2B4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11332-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, RIP, ELISA

Synonyms

Translation initiation factor eIF2B subunit delta, EIF2Bdelta, EIF2BD, eIF2B GDP-GTP exchange factor subunit delta, EIF2B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHCIF/ICC

WB analysis using 11332-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11332-1-AP (EIF2B4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 11332-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11332-1-AP (EIF2B4 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 11332-1-AP
Immunohistochemical analysis of paraffin-embedded human testis slide using 11332-1-AP (EIF2B4 Antibody) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 11332-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 11332-1-AP (EIF2B4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	MCF-7 cells, K-562 cells, HeLa cells, HL-60 cells, mouse skeletal muscle tissue
Positive IHC detected in	human breast cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

11332-1-AP targets EIF2B4 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1875 Product name: Recombinant human EIF2B4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-341 aa of BC001870 Sequence: MAAVAVAVREDSGSGMKAELPPGPGAVGREMTKEEKLQLRKEKKQQKKKRKEEKGAEPETGSAVSAAQCQVGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQARKGEQGGPPPKASPSTAGETPSGVKRLPEYPQVDDLLLRRLVKKPERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQFMSIPSSVIHPAMVRLGLQYSQGLVSGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVGSSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSSL Predict reactive species
Full Name	eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa
Calculated Molecular Weight	523 aa, 58 kDa
Observed Molecular Weight	58 kDa
GenBank Accession Number	BC001870
Gene Symbol	EIF2B4
Gene ID (NCBI)	8890
RRID	AB_2277640
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UI10
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IF protocol for EIF2B4 antibody 11332-1-AP	Download protocol
IHC protocol for EIF2B4 antibody 11332-1-AP	Download protocol
WB protocol for EIF2B4 antibody 11332-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Mol Cell The HRI branch of the integrated stress response selectively triggers mitophagy Authors - Yogaditya Chakrabarty KD Validated View Article
human	WB	Nat Commun Sugar phosphate activation of the stress sensor eIF2B. Authors - Qi Hao View Article
human	WB	Elife Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response. Authors - Carmela Sidrauski View Article
	WB	Elife The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes. Authors - Yao Liang Wong View Article
human	WB	ACS Cent Sci Comparative Nucleotide-Dependent Interactome Analysis Reveals Shared and Differential Properties of KRas4a and KRas4b. Authors - Xiaoyu Zhang View Article
human	WB	Elife eIF2B conformation and assembly state regulate the integrated stress response. Authors - Michael Schoof View Article