Tested Applications
| Positive WB detected in | human lung tissue, Jurkat cells, mouse lung tissue |
| Positive IHC detected in | human ovary tumor tissue, human colon cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11536-1-AP targets PREP in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2101 Product name: Recombinant human PREP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 360-710 aa of BC030636 Sequence: ILQLHDLTTGALLKTFPLDVGSIVGYSGQKKDTEIFYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDYQTVQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRHMGGILAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTSPKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKYTIGHAWTTDYGCSDSKQHFEWLVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNVDWIP Predict reactive species |
| Full Name | prolyl endopeptidase |
| Calculated Molecular Weight | 710 aa, 81 kDa |
| Observed Molecular Weight | 81 kDa |
| GenBank Accession Number | BC030636 |
| Gene Symbol | PREP |
| Gene ID (NCBI) | 5550 |
| RRID | AB_2284249 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48147 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PREP, also named as PE and PEP, belongs to the peptidase S9A family. It cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Together with MMPs, PREP may play an important role in the formation of PGP and thus in the pathophysiology of lung emphysema.PREP is the only enzyme directly capable of cleaving PGP from shorter portions of collagen.(PMID:21112944)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PREP antibody 11536-1-AP | Download protocol |
| IHC protocol for PREP antibody 11536-1-AP | Download protocol |
| WB protocol for PREP antibody 11536-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody (catalog #11536-1-AP) has 9 total citations published in journals including Gut, J Exp Clin Cancer Res, Cell Death Discov, Int J Biol Macromol, Oxid Med Cell Longev, J Gen Virol, Am J Physiol Lung Cell Mol Physiol, Fitoterapia, and PLoS One. Research fields span inflammatory bowel disease, colorectal liver metastasis, bone regeneration, Alzheimer's disease (Aβ42), intervertebral disc degeneration, virology, lung emphysema, and autophagy.
| Species | Application | Title |
|---|---|---|
Gut Collagen degradation and neutrophilic infiltration: a vicious circle in inflammatory bowel disease. | ||
J Exp Clin Cancer Res A novel role of secreted methionine adenosyltransferase α2 in colorectal liver metastases. | ||
Cell Death Discov Silencing of prolyl endopeptidase protects against bone loss and enhances regeneration via bone anabolic and anti-catabolic effects.
| ||
Int J Biol Macromol A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin. | ||
Oxid Med Cell Longev Increased Expression of Prolyl Endopeptidase Induced by Oxidative Stress in Nucleus Pulposus Cells Aggravates Intervertebral Disc Degeneration.
| ||
Am J Physiol Lung Cell Mol Physiol Cigarette smoke-induced lung emphysema in mice is associated with prolyl endopeptidase, an enzyme involved in collagen breakdown. |
