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PREP Polyclonal antibody, PBS Only (11536-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of human lung using 11536-1-AP (same clone as 11536-1-PBS)
human lung tissue were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.
WB analysis of mouse lung using 11536-1-AP (same clone as 11536-1-PBS)
mouse lung tissue were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 11536-1-AP (same clone as 11536-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 11536-1-AP (same clone as 11536-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 11536-1-AP (same clone as 11536-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 11536-1-AP (same clone as 11536-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas using 11536-1-AP (PREP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.
IF Staining of A549 using 11536-1-AP (same clone as 11536-1-PBS)
Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 11536-1-AP (PREP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PREP Polyclonal antibody, PBS Only

Name: PREP Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 11536-1-PBS

PREP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 11536-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Post-proline cleaving enzyme, PEP, EC:3.4.21.26

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

11536-1-PBS targets PREP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2101 Product name: Recombinant human PREP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 360-710 aa of BC030636 Sequence: ILQLHDLTTGALLKTFPLDVGSIVGYSGQKKDTEIFYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDYQTVQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRHMGGILAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTSPKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKYTIGHAWTTDYGCSDSKQHFEWLVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNVDWIP Predict reactive species
Full Name	prolyl endopeptidase
Calculated Molecular Weight	710 aa, 81 kDa
Observed Molecular Weight	81 kDa
GenBank Accession Number	BC030636
Gene Symbol	PREP
Gene ID (NCBI)	5550
RRID	AB_2284249
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P48147
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PREP, also named as PE and PEP, belongs to the peptidase S9A family. It cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Together with MMPs, PREP may play an important role in the formation of PGP and thus in the pathophysiology of lung emphysema.PREP is the only enzyme directly capable of cleaving PGP from shorter portions of collagen.(PMID:21112944)