Tested Applications
| Positive WB detected in | mouse brain tissue, HEK-293 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11537-1-AP targets LRRC8D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2138 Product name: Recombinant human LRRC8D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 515-858 aa of BC024159 Sequence: LQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIVTIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISMIPIEIGLLQNLQHLHITGNKVDILPKQLFKCIKLRTLNLGQNCITSLPEKVGQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDHLFDTLPLEVKEALNQDINIPFANGI Predict reactive species |
| Full Name | leucine rich repeat containing 8 family, member D |
| Calculated Molecular Weight | 858 aa, 98 kDa |
| Observed Molecular Weight | 98-120 kDa |
| GenBank Accession Number | BC024159 |
| Gene Symbol | LRRC8D |
| Gene ID (NCBI) | 55144 |
| RRID | AB_2137767 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7L1W4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LRRC8D (or LRRC5L) gene encodes a 98-kDa multi-pass membrane protein leucine-rich repeat-containing protein 8D, which contains 13 LRRs (leucine-rich repeats). The molecular function of this protein is so far not well understood. Recently, LRRC8D gene was found to be up-regulated after IL-2 deprivation in immortalised Interleukin-2(IL-2)-dependent human T cells, indicating a possible involvement of LRRC8D in cancer development. This LRRC8D antibody can't cross-recognize LRRC8A.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LRRC8D antibody 11537-1-AP | Download protocol |
| IHC protocol for LRRC8D antibody 11537-1-AP | Download protocol |
| WB protocol for LRRC8D antibody 11537-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LRRC8D Polyclonal antibody (Cat# 11537-1-AP) has a total of 5 citations published in Cell Genom, Cells, Communications Biology, American Journal of Physiology – Cell Physiology, and Cell Physiology and Biochemistry. Research fields span neurobiology (autism spectrum disorder, GABAergic signaling in neural stem cells), ion channel biology (LRRC8-mediated volume-regulated anion channel permeability), platinum drug uptake mechanisms, and epidermal cell volume regulation. Applications cited include WB, IF, and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Genom Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders | ||
Cells Lateral Ventricular Neural Stem Cells Provide Negative Feedback to Circuit Activation Through GABAergic Signaling | ||
Am J Physiol Cell Physiol Subunit-specific roles of LRRC8 proteins in determining glutamate permeability of astrocytic volume-regulated anion channels.
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