Tested Applications
| Positive WB detected in | HCT 116 cells, HepG2 cells |
| Positive IHC detected in | human colon cancer tissue, mouse liver tissue, mouse kidney tissue, human pancreas cancer tissue, rat pancreas tissue, human placenta tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11630-1-AP targets MIG6; ERRFI1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2202 Product name: Recombinant human MIG6; ERRFI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 113-462 aa of BC025337 Sequence: CGFKKLTVNGVCASTPPLTPIKNSPSLFPCAPLCERGSRPLPPLPISEALSLDDTDCEVEFLTSSDTDFLLEDSTLSDFKYDVPGRRSFRGCGQINYAYFDTPAVSAADLSYVSDQNGGVPDPNPPPPQTHRRLRRSHSGPAGSFNKPAIRISNCCIHRASPNSDEDKPEVPPRVPIPPRPVKPDYRRWSAEVTSSTYSDEDRPPKVPPREPLSPSNSRTPSPKSLPSYLNGVMPPTQSFAPDPKYVSSKALQRQNSEGSASKVPCILPIIENGKKVSSTHYYLLPERPPYLDKYEKFFREAEETNGGAQIQPLPADCGISSATEKPDSKTKMDLGGHVKRKHLSYVVSP Predict reactive species |
| Full Name | ERBB receptor feedback inhibitor 1 |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 51 kDa |
| GenBank Accession Number | BC025337 |
| Gene Symbol | MIG6 |
| Gene ID (NCBI) | 54206 |
| RRID | AB_2101525 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJM3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MIG6; ERRFI1 antibody 11630-1-AP | Download protocol |
| IHC protocol for MIG6; ERRFI1 antibody 11630-1-AP | Download protocol |
| WB protocol for MIG6; ERRFI1 antibody 11630-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MIG6/ERRFI1 polyclonal antibody (Cat# 11630-1-AP) has 23 citations in journals including Nature Biomedical Engineering, Cell Discovery, PNAS, EMBO Reports, Nucleic Acids Research, Cancer Letters, and JCI Insight. Research fields cover oncology (NSCLC, breast, prostate, liver, glioma), EGFR/RAS/ERK and PI3K/AKT signaling, bone and cartilage development, photoaging, diabetes, endometriosis, and epigenetic regulation.
| Species | Application | Title |
|---|---|---|
Nat Biomed Eng Profiling of protein-protein interactions via single-molecule techniques predicts the dependence of cancers on growth-factor receptors. | ||
Cell Discov RNA-binding protein SAMD4 regulates skeleton development through translational inhibition of Mig6 expression. | ||
Nucleic Acids Res A genome landscape of SRSF3-regulated splicing events and gene expression in human osteosarcoma U2OS cells. | ||
Cancer Lett MIG6 loss increased RET inhibitor tolerant persister cells in RET-rearranged non-small cell lung cancer | ||
Cell Death Discov ERRFI1 induces apoptosis of hepatocellular carcinoma cells in response to tryptophan deficiency.
| ||
Proc Natl Acad Sci U S A Cartilage-specific deletion of Mig-6 results in osteoarthritis-like disorder with excessive articular chondrocyte proliferation. |
Reviews
What customers say
The single review for this product is a 5-star rating. The user applied it for immunohistochemistry on cryostat slices from mouse brain at a 1:500 dilution and reported that it "worked exactly as desired." Overall, the feedback is positive, indicating reliable performance in IHC applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anna (Verified Customer) (09-19-2025) | Worked exactly as desired.
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