Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11772-1-AP targets SFRS6 in WB, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2412 Product name: Recombinant human SFRS6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-344 aa of BC006832 Sequence: MPRVYIGRLSYNVREKDIQRFFSGYGRLLEVDLKNGYGFVEFEDSRDADDAVYELNGKELCGERVIVEHARGPRRDRDGYSYGSRSGGGGYSSRRTSGRDKYGPPVRTEYRLIVENLSSRCSWQDLKDFMRQAGEVTYADAHKERTNEGVIEFRSYSDMKRALDKLDGTEINGRNIRLIEDKPRTSHRRSYSGSRSRSRSRRRSRSRSRRSSRSRSRSISKSRSRSRSRSKGRSRSRSKGRKSRSKSKSKPKSDRGSHSHSRSRSKDEYEKSRSRSRSRSPKENGKGDIKSKSRSRSQSRSNSPLPVPPSKARSVSPPPKRATSRSRSRSRSKSRSRSRSSSRD Predict reactive species |
| Full Name | splicing factor, arginine/serine-rich 6 |
| Calculated Molecular Weight | 344 aa, 40 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC006832 |
| Gene Symbol | SFRS6 |
| Gene ID (NCBI) | 6431 |
| RRID | AB_2185212 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13247 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SR proteins have a characteristic C-terminal Ser/Arg-rich repeat (RS domain) of variable length and constitute a family of highly conserved nuclear phosphoproteins that can function as both essential and alternative pre-mRNA splicing factors [PMID:7556075]. SRFS6 has a pivotal role in the TF exon 5 definition, an event essential for flTF biosynthesis. Besides, up- or down regulation of SRSF6 alters the splicing of several tumor suppressors and oncogenes to generate the oncogenic isoforms and reduce the tumor suppressive isoforms [PMID:23132731].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SFRS6 antibody 11772-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SFRS6 Polyclonal antibody (Cat# 11772-1-AP) has 6 citations published in Nature Communications, Communications Biology, Journal of Cellular and Molecular Medicine, iScience, Gene, and Data Brief. Research fields span splicing regulation in tumor growth, ovarian cancer progression, chronic renal fibrosis, adipocyte thermogenesis and energy expenditure, aging biomarkers, and multi-tissue transcriptomics in klotho mice.
| Species | Application | Title |
|---|---|---|
Nat Commun Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth. | ||
Commun Biol IGF2BP3 recruits NUDT21 to regulate SPTBN1 alternative polyadenylation and drive ovarian cancer progression | ||
J Cell Mol Med Renal Tubular Epithelial Cell-Derived hsa_circ_0008925 From Urine Is Related to Chronic Renal Fibrosis | ||
iScience Adipocyte-specific Steap4 deficiency reduced thermogenesis and energy expenditure in mice | ||
Gene Intron retention as a new pre-symptomatic marker of aging and its recovery to the normal state by a traditional Japanese multi-herbal medicine. | ||
Data Brief Data of RNA-seq transcriptomes of liver, bone, heart, kidney and blood in klotho mice at a pre-symptomatic state and the effect of a traditional Japanese multi-herbal medicine, juzentaihoto. |
Reviews
What customers say
One reviewer (Jingwen Yin) gave this product a 5-star rating for Immunofluorescence on human cancer cell lines. Using a 1:150 dilution with lot 00002378, they reported that "the beautiful staining signal showed up inside the nucleus," indicating strong and specific nuclear staining performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jingwen (Verified Customer) (04-28-2021) | The beautiful staining signal showed up inside the nucleus.
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