Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12029-1-AP targets POP1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2660 Product name: Recombinant human POP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC011529 Sequence: MSNAKERKHAKKMRNQPTNVTLSSGFVADRGVKHHSGGEKPFQAQKQEPHPGTSRQRQTRVNPHSLPDPEVNEQSSSKGMFRKKGGWKAGPEGTSQEIPKYITASTFAQARAAEISAMLKAVTQKSSNSLVFQTLPRHMRRRAMSHNVKRLPRRLQEIAQKEAEKAVHQKKEHSKNKCHKARRCHMNRTLEFNRRQKKNIWLETHIWHAKRFHMVKKWGYCLGERPTVKSHRACYRAMTNRCLLQDLSYYCCLELKGKEEEILKALSGMCNIDTGLTFAAVHCLSGKRQGSLVLYRVNKYPREMLGPVTFIWKSQRTPGDPSESRQLWIWLHPTLKQDILEEIKAACQCV Predict reactive species |
| Full Name | processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae) |
| Calculated Molecular Weight | 1024 aa, 115 kDa |
| Observed Molecular Weight | 115 kDa |
| GenBank Accession Number | BC011529 |
| Gene Symbol | POP1 |
| Gene ID (NCBI) | 10940 |
| RRID | AB_2166477 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99575 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Processing of Precursor 1 (POP1), a core component of RNase subunits, is an autoantigen in patients with connective tissue disease and is involved in suppressing inflammation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for POP1 antibody 12029-1-AP | Download protocol |
| IHC protocol for POP1 antibody 12029-1-AP | Download protocol |
| IP protocol for POP1 antibody 12029-1-AP | Download protocol |
| WB protocol for POP1 antibody 12029-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
Research (Wash D C) POP1 Facilitates Proliferation in Triple-Negative Breast Cancer via m6A-Dependent Degradation of CDKN1A mRNA | ||
Cell Rep m1A and m6A modifications function cooperatively to facilitate rapid mRNA degradation
|
Reviews
What customers say
Most reviewers report positive results with this antibody for Western Blot at a 1:500 dilution. Two users confirmed strong performance in mouse lung tumor cells and tissues, noting clear and reliable detection. One reviewer experienced multiple non-specific bands when testing HepG2 cell lysate, attributing the issue to the absence of a protease inhibitor during sample preparation. Overall, the antibody performs well in mouse systems, while users working with human cell lines may benefit from adding protease inhibitors to improve specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jim (Verified Customer) (02-13-2026) | The antibody works well in mice.
|
Jim (Verified Customer) (10-20-2025) | Worked well in mouse lung tumor tissues for WB.
|
Estefania (Verified Customer) (05-16-2022) | Many bands appearing. Protease inhibitor not used for the preparation of cell lysate.
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