Tested Applications
| Positive WB detected in | mouse liver tissue, mouse kidney tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12239-1-AP targets CBY1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2882 Product name: Recombinant human CBY1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 41-126 aa of BC016139 Sequence: GSPTMNLAGQSLKFENGQWIAETGVSGGVDRREVQRLRRRNQQLEEENNLLRLKVDILLDMLSESTAESHLMEKELDELRISRKRK Predict reactive species |
| Full Name | chibby homolog 1 (Drosophila) |
| Calculated Molecular Weight | 126 aa, 14 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC016139 |
| Gene Symbol | CBY1 |
| Gene ID (NCBI) | 25776 |
| RRID | AB_10951858 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3M2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CBY1 antibody 12239-1-AP | Download protocol |
| IP protocol for CBY1 antibody 12239-1-AP | Download protocol |
| WB protocol for CBY1 antibody 12239-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CBY1 Polyclonal antibody (Cat# 12239-1-AP) has 7 citations published in Advanced Science, Journal of Cell Biology, PeerJ, Medicine (Baltimore), Developmental Dynamics, medRxiv, and Heliyon. Research fields span ciliary biology, airway cell differentiation, endoplasmic reticulum stress in cancer, laryngeal squamous cell carcinoma, cardiac valve development, ovarian cancer, and EMT/cisplatin resistance. Applications cited include WB, IHC, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) The ARPKD Protein DZIP1L Regulates Ciliary Protein Entry by Modulating the Architecture and Function of Ciliary Transition Fibers | ||
J Cell Biol Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation. | ||
PeerJ Multi-omics evidence reveals a causal role of endoplasmic reticulum stress in cancer development. | ||
Medicine (Baltimore) Upregulated TC1 and downregulated Chibby were correlated with the aberrant β-catenin expression in laryngeal squamous cell carcinoma | ||
Dev Dyn DZIP1 regulates mammalian cardiac valve development through a Cby1-β-catenin mechanism. | ||
Heliyon MAP7 drives EMT and cisplatin resistance in ovarian cancer via wnt/β-catenin signaling |
Reviews
What customers say
The single available review for this product is a 5-star rating from a Western Blot application using HEK293T cells at a 1/1000 dilution. The reviewer did not leave written comments or upload an image, but the top rating indicates strong satisfaction with the antibody's performance under those conditions. Overall, the limited review base suggests the product is well-received, though broader user feedback is not yet available.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Feng-Qian (Verified Customer) (12-03-2018) |
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