Tested Applications
| Positive WB detected in | Y79 cells, human ileum tissue, LNCaP cells, mouse small intestine tissue, rat small intestine tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12262-1-AP targets ETFA in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2931 Product name: Recombinant human ETFA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-333 aa of BC015526 Sequence: MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPVEISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILKKK Predict reactive species |
| Full Name | electron-transfer-flavoprotein, alpha polypeptide |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC015526 |
| Gene Symbol | ETFA |
| Gene ID (NCBI) | 2108 |
| RRID | AB_2100540 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13804 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kD alpha subunits (ETFA) and 28 kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. ETFA serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ETFA antibody 12262-1-AP | Download protocol |
| IF protocol for ETFA antibody 12262-1-AP | Download protocol |
| IHC protocol for ETFA antibody 12262-1-AP | Download protocol |
| WB protocol for ETFA antibody 12262-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ETFA antibody (Cat# 12262-1-AP) has 12 total citations published in journals including Redox Biology, Molecular Cell, Developmental Cell, Cell Molecular Life Sciences, Bioorganic Chemistry, FASEB Journal, Journal of Proteome Research, Animal Bioscience, Journal of Proteomics, Proteome Science, and bioRxiv. Research fields span ferroptosis, apoptosis, muscle atrophy, lung cancer, cardiac hypertrophy, one-carbon metabolism, gut microbiota, and proteomics.
| Species | Application | Title |
|---|---|---|
Redox Biol Txnrd2 loss in skeletal muscle causes muscle atrophy and drives leanness and obesity resistance. | ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. | ||
Cell Mol Life Sci Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism. | ||
Bioorg Chem A pomalidomide-based gefitinib PROTAC degrader effectively inhibits lung cancer progression in EGFR-TKIs-acquired resistant models by targeting EGFR degradation and ETFA-mediated ATP generation. | ||
Bioorg Chem Pomalidomide sensitizes lung cancer cells to TRAIL/CDDP-induced apoptosis via directly targeting electron transfer flavoprotein alpha subunit |
