Tested Applications
| Positive WB detected in | A2780 cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12409-1-AP targets APPBP2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3092 Product name: Recombinant human APPBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 288-586 aa of BC018121 Sequence: SDTLLDYGFYLLNVDNICQSVAIYQAALDIRQSVFGGKNIHVATAHEDLAYSSYVHQYSSGKFDNALFHAERAIGIITHILPEDHLLLASSKRVKALILEEIAIDCHNKETEQRLLQEAHDLHLSSLQLAKKAFGEFNVQTAKHYGNLGRLYQSMRKFKEAEEMHIKAIQIKEQLLGQEDYEVALSVGHLASLYNYDMNQYENAEKLYLRSIAIGKKLFGEGYSGLEYDYRGLIKLYNSIGNYEKVFEYHNVLSNWNRLRDRQYSVTDALEDVSTSPQSTEEVVQSFLISQNVEGPSC Predict reactive species |
| Full Name | amyloid beta precursor protein (cytoplasmic tail) binding protein 2 |
| Calculated Molecular Weight | 585 aa, 67 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | BC018121 |
| Gene Symbol | APPBP2 |
| Gene ID (NCBI) | 10513 |
| RRID | AB_2918029 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92624 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Amyloid proteinbinding protein 2 (APPBP2) is a hydrophilic tubulin that interacts with microtubules and is functionally involved in beta-amyloid precursor protein (APP) transport and/or processing. Based on the regulation of microtubules and APP, APPBP2 is found to be involved in the oncogenesis of various types of cancers, such as breast cancer, ovarian clear cell adenocarcinomas, desmoplastic medulloblastomas and neuroblastomas. Recently, Studies have demonstrated that APPBP2 is a potential target for diagnosis and therapeutic intervention in non-small cell lung cancer (NSCLC) (PMID: 31105033).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for APPBP2 antibody 12409-1-AP | Download protocol |
| WB protocol for APPBP2 antibody 12409-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The APPBP2 Polyclonal antibody (Cat# 12409-1-AP) has 1 citation. It was used in a Western blot study published in Diabetes (2023), examining how PRMT4 facilitates white adipose tissue browning and thermogenesis by methylating PPARγ. The research falls within the fields of metabolic regulation, adipose tissue biology, and diabetes. The cited application was WB in mouse samples.
| Species | Application | Title |
|---|---|---|
Diabetes PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ |
Reviews
What customers say
The single review for this antibody (rated 3/5) was from a Western Blot application on HEK293T cells at a 1:1000 dilution. The reviewer noted that the antibody produces multiple bands with considerable background, and the target protein is not the most distinct band. However, they still considered it the best antibody they had found for APPBP2, as it does appear to detect the correct protein despite the suboptimal specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anna (Verified Customer) (02-05-2026) | Produces many bands but does seem to report on the correct protein (not the most distinct band, unfortunately). Despite the pretty bad background this is the best antibody I have been able to find for APPBP2.
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