PANK1/2/3 Polyclonal antibody

PANK1/2/3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12466-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

hPanK3, PANK1/2/3, PANK3, pantothenate kinase 3, Pantothenic acid kinase 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of HepG2 using 12466-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12466-1-AP (PANK1/2/3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human hepatocirrhosis using 12466-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human hepatocirrhosis using 12466-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 12466-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 12466-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 12466-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 12466-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 12466-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 12466-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12466-1-AP (PANK1/2/3 antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells
Positive IHC detected in	human hepatocirrhosis tissue, human breast cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12466-1-AP targets PANK1/2/3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3140 Product name: Recombinant human PANK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 61-370 aa of BC013705 Sequence: TGIRDVHLELKDLTLFGRRGNLHFIRFPTQDLPTFIQMGRDKNFSTLQTVLCATGGGAYKFEKDFRTIGNLHLHKLDELDCLVKGLLYIDSVSFNGQAECYYFANASEPERCQKMPFNLDDPYPLLVVNIGSGVSILAVHSKDNYKRVTGTSLGGGTFLGLCSLLTGCESFEEALEMASKGDSTQADKLVRDIYGGDYERFGLPGWAVASSFGNMIYKEKRESVSKEDLARATLVTITNNIGSVARMCAVNEKINRVVFVGNFLRVNTLSMKLLAYALDYWSKGQLKALFLEHEGYFGAVGALLGLPNFS Predict reactive species
Full Name	pantothenate kinase 3
Calculated Molecular Weight	370 aa, 41 kDa
Observed Molecular Weight	41 kDa
GenBank Accession Number	BC013705
Gene Symbol	PANK3
Gene ID (NCBI)	79646
RRID	AB_10644117
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H999
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for PANK1/2/3 antibody 12466-1-AP	Download protocol
WB protocol for PANK1/2/3 antibody 12466-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The PANK1/2/3 Polyclonal antibody (Cat# 12466-1-AP) has 1 total citation. It appears in the International Journal of Molecular Sciences (IF 5.6), published in December 2022. The cited study investigated how MicroRNA-200c affects milk fat synthesis by targeting PANK3 in ovine mammary epithelial cells, placing the research in the fields of molecular biology, lipid metabolism, and mammary gland physiology. The antibody was used for Western blot analysis in sheep samples.

Species	Application	Title
sheep	WB	Int J Mol Sci MicroRNA-200c Affects Milk Fat Synthesis by Targeting PANK3 in Ovine Mammary Epithelial Cells Authors - Zhiyun Hao View Article