Tested Applications
| Positive WB detected in | THP-1 cells |
| Positive IP detected in | THP-1 cells |
| Positive IHC detected in | rat testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12622-1-AP targets RFX2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3310 Product name: Recombinant human RFX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 424-723 aa of BC028579 Sequence: VLPKDKLISLCQCDPILRWMRSCDHILYQALVEILIPDVLRPVPSTLTQAIRNFAKSLEGWLTNAMSDFPQQVIQTKVGVVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASWVCQCEESVVQRLEQDFKLTLQQQSSLDQWASWLDSVVTQVLKQHAGSPSFPKAAQQFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMFYLVEHRVAEATGETPIAVMGEFNDLASLSLTLLDKDDMGDEQRGSEAGPDARSLGEPLVKRERSDPNHSLQGI Predict reactive species |
| Full Name | regulatory factor X, 2 (influences HLA class II expression) |
| Calculated Molecular Weight | 723 aa, 80 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC028579 |
| Gene Symbol | RFX2 |
| Gene ID (NCBI) | 5990 |
| RRID | AB_3741832 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48378 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RFX2 is a key regulator of ciliogenesis in vertebrates, and knockdown of Rfx2 causes defects in neural tube closure and in left-right axis patterning (PMID: 26248850). RFX2 expression is greatly enhanced in adult testis and that RFX2 is equally prominent in highly enriched populations of late pachytene spermatocytes and round spermatids(PMID: 22227339). RFX2 is also required for the development of left-right asymmetry, and motile cilia on RFX2-expressing cells are sufficient to establish adequate fluid flow across the COA to direct normal LR development in mouse (PMID: 22233545).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RFX2 antibody 12622-1-AP | Download protocol |
| IP protocol for RFX2 antibody 12622-1-AP | Download protocol |
| WB protocol for RFX2 antibody 12622-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RFX2 Polyclonal antibody (Cat# 12622-1-AP) has 1 total citation. It was cited in PLoS One (2022) in a study investigating nintedanib-induced gene expression changes in the lung of rheumatoid arthritis-associated interstitial lung disease mice. The antibody was used for WB and IHC applications in mouse samples, placing its cited use in the fields of pulmonary fibrosis and rheumatoid arthritis research.
| Species | Application | Title |
|---|---|---|
PLoS One Nintedanib induces gene expression changes in the lung of induced-rheumatoid arthritis-associated interstitial lung disease mice. |
