Tested Applications
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12829-1-AP targets SPACA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3549 Product name: Recombinant human SPACA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-229 aa of BC029488 Sequence: SARGTNVTAAVQDAGLAHEGEGEEETENNDSETAENYAPPETEDVSNRNVVKEVEFGMCTVTCGIGVREVILTNGCPGGESKCVVRVEECRGPTDCGWGKPISESLESVRLACIHTSPLNRFKYMWKLLRQDQQSIILVNDSAILEVRKESHPLAFECDTLDNNEIVATIKFTVYTSSELQMRRSSLPATDAALIFVLTIGVII Predict reactive species |
| Full Name | sperm acrosome associated 1 |
| Calculated Molecular Weight | 294 aa, 32 kDa |
| GenBank Accession Number | BC029488 |
| Gene Symbol | SPACA1 |
| Gene ID (NCBI) | 81833 |
| RRID | AB_2877886 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBV2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SPACA1, also named as SAMP32, is a membrane protein that localizes in the equatorial segment of spermatozoa in mammals and is reported to function in sperm-egg fusion. SPACA1 is regulated by phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SPACA1 antibody 12829-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SPACA1 Polyclonal antibody (Cat# 12829-1-AP) has 4 citations published in Ecotoxicology and Environmental Safety, Cell and Molecular Life Sciences, International Journal of Molecular Sciences, and Free Radical Research. The associated research fields include sperm biology, male fertility, spermatogenesis, and environmental toxicology effects on reproduction.
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice. | ||
Int J Mol Sci Decellularized Testicular Extracellular Matrix Scaffolds Support Mature Spermatogenesis: Impact of Donor Age and Transplantation Microenvironment. | ||
Free Radic Res Heterozygous SOD1 deficiency in mice with an NZW background causes male infertility and an aberrant immune phenotype |
