Tested Applications
| Positive WB detected in | HL-60 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13181-1-AP targets RBBP5 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3848 Product name: Recombinant human RBBP5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-327 aa of BC037284 Sequence: MNLELLESFGQNYPEEADGTLDCISMALTCTFNRWGTLLAVGCNDGRIVIWDFLTRGIAKIISAHIHPVCSLCWSRDGHKLVSASTDNIVSQWDVLSGDCDQRFRFPSPILKVQYHPRDQNKVLVCPMKSAPVMLTLSDSKHVVLPVDDDSDLNVVASFDRRGEYIYTGNAKGKILVLKTDSQDLVASFRVTTGTSNTTAIKSIEFARKGSCFLINTADRIIRVYDGREILTCGRDGEPEPMQKLQDLVNRTPWKKCCFSGDGEYIVAGSARQHALYIWEKSIGNLVKILHGTRGELLLDVAWHPVRPIIASISSGVVSIWAQNQVE Predict reactive species |
| Full Name | retinoblastoma binding protein 5 |
| Calculated Molecular Weight | 538 aa, 59 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | BC037284 |
| Gene Symbol | RBBP5 |
| Gene ID (NCBI) | 5929 |
| RRID | AB_2177773 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15291 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RBQ-3, also known as RBBP5 (retinoblastoma binding protein 5) or SWD1, is a 538 amino acid protein that localizes to the nucleus and contains six WD repeats. Expressed ubiquitously, RBQ-3 functions as a component of the Set1 complex and preferentially binds to underphosphorylated forms of the retinoblastoma (Rb) protein, possibly playing a role in the regulation of cell proliferation. RBQ-3 exists as two alternatively spliced isoforms and, upon DNA damage, is subject to phosphorylation by ATM or ATR.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RBBP5 antibody 13181-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RBBP5 Polyclonal antibody (Cat# 13181-1-AP) has 1 citation. It was used in a Western Blot study published in Nature Communications (2017), investigating how Menin enhances c-Myc-mediated transcription to promote cancer progression. The associated research field is oncology, specifically transcriptional regulation in cancer.
| Species | Application | Title |
|---|---|---|
Reviews
What customers say
The product received one review with a 5-star rating for Western Blot. The reviewer reported that the antibody worked well right away, indicating reliable performance out of the box. Overall, the feedback is positive, highlighting ease of use and effective results in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Carlos (Verified Customer) (11-14-2025) | Worked well straightaway
|
